Modeling of solvent effects in the activation of the lipase from Rhizomucor miehei

被引：7

作者：

Benedetti, F

Berti, F

Linda, P

Miertus, S

Sabot, A

机构：

[1] UNIV TRIESTE,DIPARTIMENTO SCI FARMACEUT,I-34127 TRIESTE,ITALY

[2] POLYBIOS,AREA RIC,I-34012 TRIESTE,ITALY

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 1996年 / 6卷 / 07期

关键词：

D O I：

10.1016/0960-894X(96)00123-0

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

the effects of water and hydrophobic solvents on the stability of the open and closed forms of R. miehei lipase were evaluated with different solvent models. Desolvation of arginine 86 at the water-lipid interface plays a key role in the activation process, while the contribution from hydrophobic stabilization of the open form is less important. Copyright (C) 1996 Elsevier Science Ltd

引用

收藏

页码：839 / 844

页数：6

相关论文

共 9 条

[1] A SERINE PROTEASE TRIAD FORMS THE CATALYTIC CENTER OF A TRIACYLGLYCEROL LIPASE [J].

BRADY, L ;

BRZOZOWSKI, AM ;

DEREWENDA, ZS ;

DODSON, E ;

DODSON, G ;

TOLLEY, S ;

TURKENBURG, JP ;

CHRISTIANSEN, L ;

HUGEJENSEN, B ;

NORSKOV, L ;

THIM, L ;

MENGE, U .

NATURE, 1990, 343 (6260) :767-770

[2] A MODEL FOR INTERFACIAL ACTIVATION IN LIPASES FROM THE STRUCTURE OF A FUNGAL LIPASE-INHIBITOR COMPLEX [J].

BRZOZOWSKI, AM ;

DEREWENDA, U ;

DEREWENDA, ZS ;

DODSON, GG ;

LAWSON, DM ;

TURKENBURG, JP ;

BJORKLING, F ;

HUGEJENSEN, B ;

PATKAR, SA ;

THIM, L .

NATURE, 1991, 351 (6326) :491-494

[3] STRUCTURE AND ENERGETICS OF LIGAND-BINDING TO PROTEINS - ESCHERICHIA-COLI DIHYDROFOLATE REDUCTASE TRIMETHOPRIM, A DRUG-RECEPTOR SYSTEM [J].

DAUBEROSGUTHORPE, P ;

ROBERTS, VA ;

OSGUTHORPE, DJ ;

WOLFF, J ;

GENEST, M ;

HAGLER, AT .

PROTEINS-STRUCTURE FUNCTION AND GENETICS, 1988, 4 (01) :31-47

[4] CATALYSIS AT THE INTERFACE - THE ANATOMY OF A CONFORMATIONAL CHANGE IN A TRIGLYCERIDE LIPASE [J].

DEREWENDA, U ;

BRZOZOWSKI, AM ;

LAWSON, DM ;

DEREWENDA, ZS .

BIOCHEMISTRY, 1992, 31 (05) :1532-1541

[5] NEWS FROM THE INTERFACE - THE MOLECULAR-STRUCTURES OF TRIACYLGLYCERIDE LIPASES [J].

DEREWENDA, ZS ;

SHARP, AM .

TRENDS IN BIOCHEMICAL SCIENCES, 1993, 18 (01) :20-25

[6] POLARIZABLE CONTINUUM MODEL OF SOLVATION FOR BIOPOLYMERS [J].

FRECER, V ;

MIERTUS, S .

INTERNATIONAL JOURNAL OF QUANTUM CHEMISTRY, 1992, 42 (05) :1449-1468

[7] ELECTROSTATIC INTERACTION OF A SOLUTE WITH A CONTINUUM - A DIRECT UTILIZATION OF ABINITIO MOLECULAR POTENTIALS FOR THE PREVISION OF SOLVENT EFFECTS [J].

MIERTUS, S ;

SCROCCO, E ;

TOMASI, J .

CHEMICAL PHYSICS, 1981, 55 (01) :117-129

[8] THEORETICAL-STUDIES OF RHIZOMUCOR-MIEHEI LIPASE ACTIVATION [J].

NORIN, M ;

OLSEN, O ;

SVENDSEN, A ;

EDHOLM, O ;

HULT, K .

PROTEIN ENGINEERING, 1993, 6 (08) :855-863

[9]

WEST RC, 1989, HDB CHEM PHYSICS