Genome-Wide Profiling of MicroRNAs in Adipose Mesenchymal Stem Cell Differentiation and Mouse Models of Obesity

被引：50

作者：

Bengestrate, Lena ^{[1
]}

Virtue, Sam ^{[2
]}

Campbell, Mark ^{[2
]}

Vidal-Puig, Antonio ^{[2
]}

Hadaschik, Dirk ^{[1
]}

Hahn, Peter ^{[1
]}

Bielke, Wolfgang ^{[1
]}

机构：

[1] QIAGEN GmbH, Dept Epigenet, Hilden, Germany

[2] Univ Cambridge, Addenbrookes Hosp, Inst Metab Sci, Metab Res Labs, Cambridge CB2 2QQ, England

来源：

PLOS ONE | 2011年 / 6卷 / 06期

基金：

英国生物技术与生命科学研究理事会;

关键词：

GENE-EXPRESSION; ADIPOGENESIS; MICROARRAY; ADIPOCYTES; PROTEIN; TISSUE;

D O I：

10.1371/journal.pone.0021305

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

In recent years, there has been accumulating evidence that microRNAs are key regulator molecules of gene expression. The cellular processes that are regulated by microRNAs include e. g. cell proliferation, programmed cell death and cell differentiation. Adipocyte differentiation is a highly regulated cellular process for which several important regulating factors have been discovered, but still not all are known to fully understand the underlying mechanisms. In the present study, we analyzed the expression of 597 microRNAs during the differentiation of mouse mesenchymal stem cells into terminally differentiated adipocytes by real-time RT-PCR. In total, 66 miRNAs were differentially expressed in mesenchymal stem cell-derived adipocytes compared to the undifferentiated progenitor cells. To further study the regulation of these 66 miRNAs in white adipose tissue in vivo and their dependence on PPAR gamma activity, mouse models of genetically or diet induced obesity as well as a mouse line expressing a dominant negative PPAR gamma mutant were employed.

引用

页数：12

共 33 条

[1]

Genetic determinants of energy expenditure and insulin resistance in diet-induced obesity in mice [J].