Stable metal-organic frameworks containing single-molecule traps for enzyme encapsulation

被引：686

作者：

Feng, Dawei ^{[1
]}

Liu, Tian-Fu ^{[1
]}

Su, Jie ^{[2
]}

Bosch, Mathieu ^{[1
]}

Wei, Zhangwen ^{[1
]}

Wan, Wei ^{[2
]}

Yuan, Daqiang ^{[3
]}

Chen, Ying-Pin ^{[1
,4
]}

Wang, Xuan ^{[1
]}

Wang, Kecheng ^{[1
]}

Lian, Xizhen ^{[1
]}

Gu, Zhi-Yuan ^{[1
]}

Park, Jihye ^{[1
]}

Zou, Xiaodong ^{[2
]}

Zhou, Hong-Cai ^{[1
,4
]}

机构：

[1] Texas A&M Univ, Dept Chem, College Stn, TX 77843 USA

[2] Stockholm Univ, Berzelii Ctr EXSELENT Porous Mat & Inorgan & Stru, Dept Mat & Environm Chem, S-10691 Stockholm, Sweden

[3] Chinese Acad Sci, Fujian Inst Res Struct Matter, State Key Lab Struct Chem, Fuzhou 350002, Fujian, Peoples R China

[4] Texas A&M Univ, Dept Mat Sci & Engn, College Stn, TX 77842 USA

来源：

NATURE COMMUNICATIONS | 2015年 / 6卷

基金：

瑞典研究理事会;

关键词：

IMMOBILIZED ENZYMES; CATALYTIC-ACTIVITY; SURFACE-AREA; SOLVENTS; POROSITY; SERIES;

D O I：

10.1038/ncomms6979

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Enzymatic catalytic processes possess great potential in chemical manufacturing, including pharmaceuticals, fuel production and food processing. However, the engineering of enzymes is severely hampered due to their low operational stability and difficulty of reuse. Here, we develop a series of stable metal-organic frameworks with rationally designed ultra-large mesoporous cages as single-molecule traps (SMTs) for enzyme encapsulation. With a high concentration of mesoporous cages as SMTs, PCN-333(Al) encapsulates three enzymes with record-high loadings and recyclability. Immobilized enzymes that most likely undergo single-enzyme encapsulation (SEE) show smaller Km than free enzymes while maintaining comparable catalytic efficiency. Under harsh conditions, the enzyme in SEE exhibits better performance than free enzyme, showing the effectiveness of SEE in preventing enzyme aggregation or denaturation. With extraordinarily large pore size and excellent chemical stability, PCN-333 may be of interest not only for enzyme encapsulation, but also for entrapment of other nanoscaled functional moieties.

引用

页数：8

共 32 条

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Metal-adeninate vertices for the construction of an exceptionally porous metal-organic framework [J].