A gold nanoparticle-based aptamer target binding readout for ATP assay

被引：376

作者：

Wang, Jing

Wang, Lihua ^{[1
]}

Liu, Xingfen

Liang, Zhiqiang

Song, Shiping

Li, Wenxin

Li, Genxi

Fan, Chunhai

机构：

[1] Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China

[2] Nanjing Univ, Dept Biochem, Nanjing 210093, Peoples R China

[3] Nanjing Univ, Natl Key Lab Pharmaceut Biotechnol, Nanjing 210093, Peoples R China

[4] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

来源：

ADVANCED MATERIALS | 2007年 / 19卷 / 22期

关键词：

D O I：

10.1002/adma.200602256

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A gold nanoparticle-based detection strategy for ATP assays. In the absence of ATP, gold nanoparticles are not stabilized by the rigid duplex, thus they are readily aggregated by salt (solution displaying blue colors); in contrast induces a duplex-to-aptamer structural switching, liberating a random coil-like ssDNA. Gold nanoparticles are stabilized by the liberated ssDNA, showing high resistance to salt-induced aggregation (solution staying in red).

引用

页码：3943 / 3946

页数：4

共 26 条

[1]

Baker BR, 2006, J AM CHEM SOC, V128, P3138, DOI 10.1021/ja056957p

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