Characterization of starch-debranching enzymes in pea embryos

被引:27
作者
Zhu, ZP
Hylton, CM
Rössner, U
Smith, AM
机构
[1] Shanghai Inst Plant Physiol, Shanghai 200032, Peoples R China
[2] John Innes Ctr Plant Sci Res, Norwich NR4 7UH, Norfolk, England
关键词
D O I
10.1104/pp.118.2.581
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Two distinct types of debranching enzymes have been identified in developing pea (Pisum sativum L.) embryos using native gel analysis and tests of substrate preference on purified or partially purified activities. An isoamylase-like activity capable of hydrolyzing amylopectin and glycogen but not pullulan is present throughout development and is largely or entirely confined to the plastid. Activities capable of hydrolyzing pullulan are present both inside and outside of the plastid, and extraplastidial activity increases relative to the plastidial activity during development. Both types of debranching enzyme are also present in germinating embryos. We argue that debranching enzymes are likely to have a role in starch metabolism in the plastid of the developing embryo and in starch degradation during germination.
引用
收藏
页码:581 / 590
页数:10
相关论文
共 44 条
[31]   STARCH DEGRADATION IN SPINACH LEAVES - ISOLATION AND CHARACTERIZATION OF THE AMYLASES AND R-ENZYME OF SPINACH LEAVES [J].
OKITA, TW ;
PREISS, J .
PLANT PHYSIOLOGY, 1980, 66 (05) :870-876
[32]   A DEBRANCHING ENZYME DEFICIENCY IN ENDOSPERMS OF THE SUGARY-1 MUTANTS OF MAIZE [J].
PAN, D ;
NELSON, OE .
PLANT PHYSIOLOGY, 1984, 74 (02) :324-328
[33]   STUDIES ON LIMIT DEXTRINASE IN BARLEY .3. LIMIT DEXTRINASE IN DEVELOPING KERNELS [J].
SISSONS, MJ ;
LANCE, RCM ;
SPARROW, DHB .
JOURNAL OF CEREAL SCIENCE, 1993, 17 (01) :19-24
[34]   STUDIES ON LIMIT DEXTRINASE IN BARLEY .1. PURIFICATION OF MALT LIMIT DEXTRINASE AND PRODUCTION OF MONOSPECIFIC ANTIBODIES [J].
SISSONS, MJ ;
LANCE, RCM ;
SPARROW, DHB .
JOURNAL OF CEREAL SCIENCE, 1992, 16 (02) :107-116
[35]   MAJOR DIFFERENCES IN ISOFORMS OF STARCH-BRANCHING ENZYME BETWEEN DEVELOPING EMBRYOS OF ROUND-SEEDED AND WRINKLED-SEEDED PEAS (PISUM-SATIVUM-L) [J].
SMITH, AM .
PLANTA, 1988, 175 (02) :270-279
[36]  
STEUP M, 1988, BIOCH PLANTS, V14, P254
[37]   CHANGES OF A RICE DEBRANCHING ENZYME DURING SEED FORMATION AND GERMINATION [J].
TOGURI, T .
JOURNAL OF PLANT PHYSIOLOGY, 1991, 137 (05) :541-546
[38]   Importance of isoforms of starch-branching enzyme in determining the structure of starch in pea leaves [J].
Tomlinson, KL ;
Lloyd, JR ;
Smith, AM .
PLANT JOURNAL, 1997, 11 (01) :31-43
[39]   GLUCAN-PHOSPHORYLASE FORMS IN COTYLEDONS OF PISUM-SATIVUM L - LOCALIZATION, DEVELOPMENTAL-CHANGE, INVITRO TRANSLATION, AND PROCESSING [J].
VANBERKEL, J ;
CONRADSSTRAUCH, J ;
STEUP, M .
PLANTA, 1991, 185 (03) :432-439
[40]   IMMOBILIZATION OF LIGANDS FOR BIOSPECIFIC AFFINITY CHROMATOGRAPHY VIA THEIR HYDROXYL-GROUPS - CYCLOHEXAAMYLOSE-BETA-AMYLASE SYSTEM [J].
VRETBLAD, P .
FEBS LETTERS, 1974, 47 (01) :86-89