Development of mucoadhesive thiolated chitosan nanoparticles for biomedical applications

被引：152

作者：

Anitha, A. ^{[1
]}

Deepa, N. ^{[1
]}

Chennazhi, K. P. ^{[1
]}

Nair, S. V. ^{[1
]}

Tamura, H. ^{[2
]}

Jayakumar, R. ^{[1
]}

机构：

[1] Amrita Vishwa Vidyapeetham Univ, Amrita Ctr Nanosci & Mol Med, Amrita Inst Med Sci & Res Ctr, Kochi 682041, India

[2] Kansai Univ, Fac Chem Mat & Bioengn, Osaka 5648680, Japan

来源：

CARBOHYDRATE POLYMERS | 2011年 / 83卷 / 01期

关键词：

Thiolated chitosan; Nanoparticles; Thermal studies; Cytocompatibility; Drug delivery; ANTIMICROBIAL AGENT; DELIVERY; CHITIN; POLYMERS; SCAFFOLD; FILMS;

D O I：

10.1016/j.carbpol.2010.07.028

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

The main objective of this work was to develop nanoparticles (NPs) of a mucoadhesive polymer based on chitosan for biomedical applications. Here, we developed thiolated chitosan (TCS) using thioglycolic acid (TGA) and chitosan in the presence of 1-ethyl-3-3(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) as catalyst. The prepared TCS was characterized using FT-IR and the degree of thiol substitution was found out by Ellman's method. The TCS nanoparticles (TCS-NPs) were developed using ionic cross-linking reaction with pentasodium tripolyphosphate (TPP). The prepared TCS-NPs were characterized by DLS, AFM, FT-IR, TG/DTA, etc. In vitro cytocompatibility and cell uptake studies were also carried out. These studies suggest that the prepared NPs show less toxicity towards normal and cancer cells and they are easily taken up by both the normal and cancer cells. So the prepared TCS-NPs could be used for drug and gene delivery applications. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：66 / 73

页数：8

共 36 条

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Synthesis, characterization, cytotoxicity and antibacterial studies of chitosan, O-carboxymethyl and N,O-carboxymethyl chitosan nanoparticles [J].