Matrix-free methods for laser desorption/ionization mass spectrometry

被引:256
作者
Peterson, Dominic S. [1 ]
机构
[1] Los Alamos Natl Lab, Div Chem, Los Alamos, NM 87545 USA
关键词
MALDI; DIOS; laser desorption/ionization; mass spectrometry; matrix free;
D O I
10.1002/mas.20104
中图分类号
O433 [光谱学];
学科分类号
0703 ; 070302 ;
摘要
Matrix assisted laser desorption/ionization (MALDI) is a soft ionization mass spectrometric method that has become a preeminent technique in the analysis of a wide variety of compounds including polymers and proteins. The main drawback of MALDI is that it is difficult to analyze low molecular weight compounds (< 1,000 m/z) because the matrix that allows MALDI to work interferes in this mass range. In recent years there has been considerable interest in developing laser desorption/ ionization (LDI) techniques for the analysis of small molecules. This review examines the approaches to matrix-free LDI mass spectrometry including desorption/ionization on silicon (DIOS), sol-gels, and carbon-based microstructures. For the purposes of this review matrix-free methods are defined as those that do not require matrix to be mixed with the analyte and therefore does not require co-crystallization. The review will also examine mechanisms of ionization and applications of matrix-free LDI-MS. (c) 2006 Wiley Periodicals, Inc.
引用
收藏
页码:19 / 34
页数:16
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