Simultaneous analysis of hepatotoxic pyrrolizidine alkaloids and N-oxides in comfrey root by LC-ion trap mass spectrometry

被引：38

作者：

Wuilloud, JCA ^{[1
]}

Gratz, SR ^{[1
]}

Gamble, BM ^{[1
]}

Wolnik, KA ^{[1
]}

机构：

[1] US FDA, Forens Chem Ctr, Cincinnati, OH 45237 USA

来源：

ANALYST | 2004年 / 129卷 / 02期

关键词：

D O I：

10.1039/b311030c

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The purpose of the current study was to develop a LC-MSn method for the analysis of pyrrolizidine alkaloids (PAs) in comfrey. Published data presents an extensive list of PAs and their N-oxides present in comfrey. However, standards are not commercially available for any of the PAs typically present in cornfrey. Those PAs that are not stereoisomers were readily resolved on a C-18 column using a water-acetonitrile gradient as the mobile phase. The use of a selective technique, LC-MS/MS, allowed us to identify groups of PAs and their N-oxides, as well as identify the number of PAs present in each group, including those that were not completely resolved chromatographically.

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收藏

页码：150 / 156

页数：7

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