Use of the firefly luciferase gene in a barley (Hordeum vulgare) transformation system

被引：10

作者：

Harwood, WA ^{[1
]}

Ross, SM ^{[1
]}

Bulley, SM ^{[1
]}

Travella, S ^{[1
]}

Busch, B ^{[1
]}

Harden, J ^{[1
]}

Snape, JW ^{[1
]}

机构：

[1] John Innes Ctr, Norwich NR4 7UH, Norfolk, England

来源：

PLANT CELL REPORTS | 2002年 / 21卷 / 04期

关键词：

firefly luciferase gene; Hordeum vulgare; transformation;

D O I：

10.1007/s00299-002-0515-3

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Transgenic lines of the spring barley variety Golden Promise containing the firefly luciferase gene were produced by particle bombardment of immature embryos. Non-destructive analysis of luciferase gene expression was used to monitor the transformation process. This revealed that transformation efficiency, in terms of the percentage of bombarded immature embryos giving rise to transformed callus lines, was very high, up to 40%. Following the expression of the luciferase gene provided a method for the sensitive, non-destructive, real-time monitoring of gene expression throughout the transformation process. Luciferase expression could also be used to easily identify transgenic plants and to identify homozygous transgenic plants at an early stage. The production of transgenic barley by selecting for luciferase-positive material, without an additional selection system, was possible but technically difficult.

引用

页码：320 / 326

页数：7

共 24 条

[11] NONDESTRUCTIVE DETECTION OF FIREFLY LUCIFERASE (LUC) ACTIVITY IN SINGLE PLANT-CELLS USING A COOLED, SLOW-SCAN CCD CAMERA AND AN OPTIMIZED ASSAY [J].