Structure analysis of soluble proteins using electron crystallography

被引：22

作者：

Ellis, MJ ^{[1
]}

Hebert, H ^{[1
]}

机构：

[1] Karolinska Inst, Novum, Ctr Struct Biochem, S-14157 Huddinge, Sweden

来源：

MICRON | 2001年 / 32卷 / 05期

关键词：

electron crystallography; soluble protein; image processing; two-dimensional crystallization;

D O I：

10.1016/S0968-4328(00)00049-4

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

Electron crystallography as a structural determination technique has grown dramatically in use over recent years, Improvements in microscopes, equipment, practical techniques, computation facilities and image processing methods are reflected in the increasing number of near-atomic resolution structures that have been published. In this review we shall summarize the techniques involved in structure determination of soluble proteins using electron crystallography. Many soluble protein structures have been investigated in this manner over the past two decades. Here we present several examples where a variety of approaches have been used to gradually increase the information obtained. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：541 / 550

页数：10

共 90 条

[41]

Jensen GJ, 1998, EMBO J, V17, P2353, DOI 10.1017/S1431927600024983

[42] Three-dimensional structure of Brush Border Myosin-I at similar to 20 angstrom resolution by electron microscopy and image analysis [J].

Jontes, JD ;

Milligan, RA .

JOURNAL OF MOLECULAR BIOLOGY, 1997, 266 (02) :331-342

[43] 2-DIMENSIONAL CRYSTALLIZATION OF HISTIDINE-TAGGED, HIV-1 REVERSE-TRANSCRIPTASE PROMOTED BY A NOVEL NICKEL-CHELATING LIPID [J].

KUBALEK, EW ;

LEGRICE, SFJ ;

BROWN, PO .

JOURNAL OF STRUCTURAL BIOLOGY, 1994, 113 (02) :117-123

[44] ATOMIC MODEL OF PLANT LIGHT-HARVESTING COMPLEX BY ELECTRON CRYSTALLOGRAPHY [J].

KUHLBRANDT, W ;

WANG, DN ;

FUJIYOSHI, Y .

NATURE, 1994, 367 (6464) :614-621

[45] 2-DIMENSIONAL CRYSTALLIZATION OF DNA GYRASE-B SUBUNIT ON SPECIFICALLY DESIGNED LIPID MONOLAYERS [J].

LEBEAU, L ;

REGNIER, E ;

SCHULTZ, P ;

WANG, JC ;

MIOSKOWSKI, C ;

OUDET, P .

FEBS LETTERS, 1990, 267 (01) :38-42

[46] Two-dimensional crystallization on lipid layer:: A successful approach for membrane proteins [J].

Lévy, D ;

Mosser, G ;

Lambert, O ;

Moeck, GS ;

Bald, D ;

Rigaud, JL .

JOURNAL OF STRUCTURAL BIOLOGY, 1999, 127 (01) :44-52

[47] TWO-DIMENSIONAL CRYSTALS OF CHOLERA-TOXIN B-SUBUNIT RECEPTOR COMPLEXES - PROJECTED STRUCTURE AT 17-A RESOLUTION [J].

LUDWIG, DS ;

RIBI, HO ;

SCHOOLNIK, GK ;

KORNBERG, RD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (22) :8585-8588

[48] Binding and two-dimensional crystallization of streptavidin at the air/water interface via engineered Cu-IDA chelator lipids [J].

Maloney, KM ;

Schief, WR ;

Pack, DW ;

Frey, W ;

Arnold, FH ;

Vogel, V .

COORDINATION CHEMISTRY REVIEWS, 1999, 183 :3-18

[49] High-resolution icosahedral reconstruction: Fulfilling the promise of cryo-electron microscopy [J].

Mancini, EJ ;

deHaas, F ;

Fuller, SD .

STRUCTURE, 1997, 5 (06) :741-750

[50] SUBDOMAIN STRUCTURE OF LIPID-BOUND ANNEXIN-V RESOLVED BY ELECTRON IMAGE-ANALYSIS [J].

MOSSER, G ;

RAVANAT, C ;

FREYSSINET, JM ;

BRISSON, A .

JOURNAL OF MOLECULAR BIOLOGY, 1991, 217 (02) :241-245

← 1 2 3 4 5 6 7 8 9 →