Specificity of a Bacteroides thetaiotaomicron marker for human Feces

被引：66

作者：

Carson, CA

Christiansen, JM

Yampara-Iquise, H

Benson, VW

Baffaut, C

Davis, JV

Broz, RR

Kurtz, WB

Rogers, WM

Fales, WH

机构：

[1] Univ Missouri, Dept Vet Pathobiol, Columbia, MO 65211 USA

[2] Univ Missouri, Dept Agr Econ, Food & Agr Policy Res Inst, Columbia, MO 65211 USA

[3] Univ Missouri, Vet Med Diagnost Lab, Columbia, MO 65211 USA

[4] Univ Missouri, Dept Forestry, Columbia, MO 65211 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2005年 / 71卷 / 08期

关键词：

D O I：

10.1128/AEM.71.8.4945-4949.2005

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A bacterial primer set, known to produce a 542-bp amplicon specific for Bacteroides thetaiotaomicron, generated this product in PCR with 1 ng of extracted DNA from 92% of 25 human fecal samples, 100% of 20 sewage samples, and 16% of 31 dog fecal samples. The marker was not detected in 1 ng of fecal DNA from 61 cows, 35 horses, 44 pigs, 24 chickens, 29 turkeys, and 17 geese.

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收藏

页码：4945 / 4949

页数：5

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