Amide group assisted 3′-dephosphorylation of oligonucleotides synthesized on universal A-supports

被引：43

作者：

Azhayev, AV ^{[1
]}

Antopolsky, ML ^{[1
]}

机构：

[1] Univ Kuopio, Dept Pharmaceut Chem, FIN-70211 Kuopio, Finland

来源：

TETRAHEDRON | 2001年 / 57卷 / 23期

关键词：

oligonucleotides; amide group; solid supports;

D O I：

10.1016/S0040-4020(01)00409-4

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

(+/-)-3-Amino-1-(4,4'-dimethoxytriphenylmethyl)-2-propanediol was attached to succinylated alkylamino-controlled pore glass via the second amide bond. The resulting solid phase was acylated to give seven new universal solid supports, compatible with the preparation of all common types of oligodeoxyribonucleotides. These resins allow for fast elimination of the 3'-terminal phosphodiester or phosphorothioate function by ammonia in methanol at room temperature. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：4977 / 4986

页数：10

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