A critical role for complement C3d and the B cell coreceptor (CD19/CD21) complex in the inflammatory arthritis

被引:50
作者
Del Nagro, CJ
Kolla, RV
Rickert, RC [1 ]
机构
[1] Burnham Inst, Program Inflammatory Dis Res, Infect & Inflammatory Dis Ctr, La Jolla, CA 92037 USA
[2] Burnham Inst, Program Signal Transduct, La Jolla, CA 92037 USA
[3] Univ Calif San Diego, Div Biol Sci, Grad Program, La Jolla, CA 92093 USA
关键词
D O I
10.4049/jimmunol.175.8.5379
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Complement C3 cleavage products mediate the recognition and clearance of toxic or infectious agents. In addition, binding of the C3d fragment to Ag promotes B lymphocyte activation through coengagment of the BCR and complement receptor 2 (CD21). Signal augmentation is thought to be achieved through enhanced recruitment and activation of CD21-associated CD19. In this study we show, using the DBA/1 collagen-induced arthritis (CIA) model, that conjugation of C3d to heterologous type II collagen is sufficient to cause disease in the absence of the mycobacterial components of CFA. Transient depletion of C3 during the inductive phase of CIA delays and lessens the severity of disease, and DBA/1 mice deficient for coreceptor components CD19 or CD21 are not susceptible to CIA. Adoptive transfer experiments revealed that CD21 expression on either B cells or follicular dendritic cells is sufficient to acquire disease susceptibility, Although CD19(-/-) and CD21(-/-) mice produce primary Ab responses to heterologous and autologous type II collagen, they are impaired in the ability to activate T cells, form germinal centers, and produce secondary autoantibody responses. These findings indicate that binding of C3d to self-Ags can promote autoimmunity through enhanced Ag retention and presentation by follicular dendritic cells and B cells, respectively.
引用
收藏
页码:5379 / 5389
页数:11
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