Effect of Glycosylation and Additional Domains on the Thermostability of a Family 10 Xylanase Produced by Thermopolyspora flexuosa

被引：44

作者：

Anbarasan, Sasikala ^{[1
]}

Janis, Janne ^{[2
]}

Paloheimo, Marja ^{[3
]}

Laitaoja, Mikko ^{[2
]}

Vuolanto, Minna ^{[3
]}

Karimaki, Johanna ^{[1
]}

Vainiotalo, Pirjo ^{[2
]}

Leisola, Matti ^{[1
]}

Turunen, Ossi ^{[1
]}

机构：

[1] Aalto Univ, Dept Biotechnol & Chem Technol, Espoo 02015, Finland

[2] Univ Joensuu, Dept Chem, FIN-80101 Joensuu, Finland

[3] Roal Oy, Rajamaki 05201, Finland

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2010年 / 76卷 / 01期

基金：

芬兰科学院;

关键词：

CARBOHYDRATE-BINDING MODULE; NONOMURAEA-FLEXUOSA; THERMOTOGA-MARITIMA; CATALYTIC-ACTIVITY; STABILITY; INCREASES; CLONING; FUSION; THERMOPHILUM; PURIFICATION;

D O I：

10.1128/AEM.00357-09

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The effects of different structural features on the thermostability of Thermopolyspora flexuosa xylanase XYN10A were investigated. A C-terminal carbohydrate binding module had only a slight effect, whereas a polyhistidine tag increased the thermostability of XYN10A xylanase. In contrast, glycosylation at Asn26, located in an exposed loop, decreased the thermostability of the xylanase. The presence of a substrate increased stability mainly at low pH.

引用

页码：356 / 360

页数：5

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