Determination of neurotoxin 3-N-oxalyl-2,3-diaminopropionic acid and non-protein amino acids in Lathyrus sativus by precolumn derivatization with 1-fluoro-2,4-dinitrobenzene

被引：141

作者：

Wang, F

Chen, X

Chen, Q

Qin, XC

Li, ZX ^{[1
]}

机构：

[1] Lanzhou Univ, Natl Lab Appl Organ Chem, Lanzhou 730000, Peoples R China

[2] Lanzhou Univ, State Key Lab Arid Agroecol, Lanzhou 730000, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2000年 / 883卷 / 1-2期

基金：

中国国家自然科学基金;

关键词：

derivatization; LC; Lathyrus sativus; 3-N-oxalyl-2,3-diaminopropionic acid; non-protein amino acids; 1-fluoro-2,4-dinitrobenzene;

D O I：

10.1016/S0021-9673(00)00264-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid and simple method is presented for determining neuro-excitatory nonprotein amino acid 3-N-oxalyl-2,3-diaminopropionic acid (beta-ODAP) and non-protein amino acids in Lathyrus sativus. Seed and foliage extracts of Lathyrus sativus were treated with 1-fluoro-2,4-dinitrobenzene (FDNB) and a reversed-phase high-performance liquid chromatography method (RP HPLC) for the separation of the derivatives in the pmol range is reported. The RP HPLC method and a colorimetric method were compared for measuring ODAP. (C) 2000 Elsevier Science BN. All rights reserved.

引用

收藏

页码：113 / 118

页数：6

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