Simple reversed-phase liquid chromatography method for determination of tocopherols in edible plant oils

被引：160

作者：

Gliszczynska-Swiglo, A ^{[1
]}

Sikorska, E ^{[1
]}

机构：

[1] Poznan Univ Econ, Fac Commod Sci, PL-60967 Poznan, Poland

来源：

JOURNAL OF CHROMATOGRAPHY A | 2004年 / 1048卷 / 02期

关键词：

tocopherols; oils; vitamins; food analysis;

D O I：

10.1016/j.chroma.2004.07.051

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple and rapid reversed-phase high-performance liquid chromatography method for determination of alpha-, (beta + gamma), and delta-tocopherols in edible plant oils has been developed. Oils are diluted in 2-propanol and injected directly onto Symmetry C-18 column. Methanol and acetonitrile (1: 1) are used as a mobile phase. Tocopherols are detected using fluorescence detector set at excitation and emission wavelength 295 nm and 325 nm, respectively. The method is precise (R.S.D. not higher than 2.24%) and sensitive-detection limits (DL) are 8 ng/ml for gamma- and delta-tocopherols and 28 ng/ml for alpha-tocopherol; quantification limits (QL) were calculated as three times higher than DL. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：195 / 198

页数：4

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