A conserved transcriptional regulator is required for RNA-directed DNA methylation and plant development

被引：79

作者：

He, Xin-Jian ^{[1
,2
]}

Hsu, Yi-Feng ^{[1
,2
,3
]}

Zhu, Shihua ^{[4
]}

Liu, Hai-Liang ^{[1
,2
]}

Pontes, Olga ^{[5
]}

Zhu, Jianhua ^{[6
]}

Cui, Xinping ^{[7
]}

Wang, Co-Shine ^{[3
]}

Zhu, Jian-Kang ^{[1
,2
]}

机构：

[1] Univ Calif Riverside, Inst Integrat Genome Biol, Riverside, CA 92521 USA

[2] Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA

[3] Natl Chung Hsing Univ, Grad Inst Biotechnol, Taichung 40227, Taiwan

[4] Ningbo Univ, Coll Sci & Technol, Ningbo 315211, Zhejiang, Peoples R China

[5] Washington Univ, Dept Biol, St Louis, MO 63130 USA

[6] Univ Maryland, Dept Plant Sci & Landscape Architecture, College Pk, MD 20742 USA

[7] Univ Calif Riverside, Dept Stat, Riverside, CA 92521 USA

来源：

GENES & DEVELOPMENT | 2009年 / 23卷 / 23期

基金：

美国国家卫生研究院;

关键词：

RDM4; siRNA; transcriptional gene silencing; Pol II; Pol V; POLYMERASE-II; ARABIDOPSIS-THALIANA; POL-IV; PROTEIN; GLYCOSYLASE/LYASE; PATHWAYS; SUBUNIT; ROS1;

D O I：

10.1101/gad.1851809

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

RNA-directed DNA methylation (RdDM) is a conserved mechanism for epigenetic silencing of transposons and other repetitive elements. We report that the rdm4 (RNA-directed DNA Methylation4) mutation not only impairs RdDM, but also causes pleiotropic developmental defects in Arabidopsis. Both RNA polymerase II (Pol II)- and Pol V-dependent transcripts are affected in the rdm4 mutant. RDM4 encodes a novel protein that is conserved from yeast to humans and interacts with Pol II and Pol V in plants. Our results suggest that RDM4 functions in epigenetic regulation and plant development by serving as a transcriptional regulator for RNA Pol V and Pol II, respectively.

引用

收藏

页码：2717 / 2722

页数：6

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