Transcriptome Complexity in a Genome-Reduced Bacterium

被引：329

作者：

Gueell, Marc ^{[2
]}

van Noort, Vera ^{[1
]}

Yus, Eva ^{[2
]}

Chen, Wei-Hua ^{[1
]}

Leigh-Bell, Justine ^{[2
]}

Michalodimitrakis, Konstantinos ^{[2
]}

Yamada, Takuji ^{[1
]}

Arumugam, Manimozhiyan ^{[1
]}

Doerks, Tobias ^{[1
]}

Kuehner, Sebastian ^{[1
]}

Rode, Michaela ^{[1
]}

Suyama, Mikita ^{[1
]}

Schmidt, Sabine ^{[1
]}

Gavin, Anne-Claude ^{[1
]}

Bork, Peer ^{[1
]}

Serrano, Luis ^{[2
,3
]}

机构：

[1] EMBL, Heidelberg, Germany

[2] Univ Pompeu Fabra, CRG, Barcelona, Spain

[3] Inst Catalana Recerca & Estudis Avancats, Barcelona, Spain

来源：

SCIENCE | 2009年 / 326卷 / 5957期

基金：

欧洲研究理事会;

关键词：

NATURAL ANTISENSE TRANSCRIPTS; ESCHERICHIA-COLI; IDENTIFICATION; EXPRESSION; GENES;

D O I：

10.1126/science.1176951

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

To study basic principles of transcriptome organization in bacteria, we analyzed one of the smallest self-replicating organisms, Mycoplasma pneumoniae. We combined strand-specific tiling arrays, complemented by transcriptome sequencing, with more than 252 spotted arrays. We detected 117 previously undescribed, mostly noncoding transcripts, 89 of them in antisense configuration to known genes. We identified 341 operons, of which 139 are polycistronic; almost half of the latter show decaying expression in a staircase-like manner. Under various conditions, operons could be divided into 447 smaller transcriptional units, resulting in many alternative transcripts. Frequent antisense transcripts, alternative transcripts, and multiple regulators per gene imply a highly dynamic transcriptome, more similar to that of eukaryotes than previously thought.

引用

页码：1268 / 1271

页数：4

共 25 条

[1] Whole-Genome Tiling Array Analysis of Mycobacterium leprae RNA Reveals High Expression of Pseudogenes and Noncoding Regions [J].