The lipid phosphatase myotubularin is essential for skeletal muscle maintenance but not for myogenesis in mice

被引:162
作者
Buj-Bello, A
Laugel, V
Messaddeq, N
Zahreddine, H
Laporte, J
Pellissiert, JF
Mandel, JL
机构
[1] Univ Strasbourg 1, CNRS, INSERM, Inst Genet & Biol Mol & Cellulaire, F-670404 Illkirch Graffenstaden, France
[2] Fac Med Marseille, Unite Propre Rech Enseignement Super EA 3281, Lab Biopathol Nerveuse & Musculaire, F-13385 Marseille, France
关键词
D O I
10.1073/pnas.212498399
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Myotubularin is a ubiquitously expressed phosphatase that acts on phosphatidylinositol 3-monophosphate [PI(3)P], a lipid implicated in intracellular vesicle trafficking and autophagy. It is encoded by the MTM1 gene, which is mutated in X-linked myotubular myopathy (XLMTM), a muscular disorder characterized by generalized hypotonia and muscle weakness at birth leading to early death of most affected males. The disease was proposed to result from an arrest in myogenesis, as the skeletal muscle from patients contains hypotrophic fibers with centrally located nuclei that resemble fetal myotubes. To understand the physiopathological mechanism of XLMTM, we have generated mice lacking myotubularin by homologous recombination. These mice are viable, but their lifespan is severely reduced. They develop a generalized and progressive myopathy starting at around 4 weeks of age, with amyotrophy and accumulation of central nuclei in skeletal muscle fibers leading to death at 6-14 weeks. Contrary to expectations, we show that muscle differentiation in knockout mice occurs normally. We provide evidence that fibers with centralized myonuclei originate mainly from a structural maintenance defect affecting myotubularin-deficient muscle rather than a regenerative process. In addition, we demonstrate, through a conditional gene-targeting approach, that skeletal muscle is the primary target of murine XLMTM pathology. These mutant mice represent animal models for the human disease and will be a valuable tool for understanding the physiological role of myotubularin.
引用
收藏
页码:15060 / 15065
页数:6
相关论文
共 47 条
[21]   Characterization of mutations in fifty North American patients with X-linked myotubular myopathy [J].
Herman, GE ;
Kopacz, K ;
Zhao, W ;
Mills, PL ;
Metzenberg, A ;
Das, S .
HUMAN MUTATION, 2002, 19 (02) :114-121
[22]   Characterization of the myotubularin dual specificity phosphatase gene family from yeast to human [J].
Laporte, J ;
Blondeau, F ;
Buj-Bello, A ;
Tentler, D ;
Kretz, C ;
Dahl, N ;
Mandel, JL .
HUMAN MOLECULAR GENETICS, 1998, 7 (11) :1703-1712
[23]  
Laporte J, 2000, HUM MUTAT, V15, P393, DOI 10.1002/(SICI)1098-1004(200005)15:5<393::AID-HUMU1>3.0.CO
[24]  
2-R
[25]   A gene mutated in X-linked myotubular myopathy defines a new putative tyrosine phosphatase family conserved in yeast [J].
Laporte, J ;
Hu, LJ ;
Kretz, C ;
Mandel, JL ;
Kioschis, P ;
Coy, JF ;
Klauck, SM ;
Poustka, A ;
Dahl, N .
NATURE GENETICS, 1996, 13 (02) :175-182
[26]   Diagnosis of X-linked myotubular myopathy by detection of myotubularin [J].
Laporte, J ;
Kress, W ;
Mandel, JL .
ANNALS OF NEUROLOGY, 2001, 50 (01) :42-46
[27]   The myotubularin family: from genetic disease to phosphoinositide metabolism [J].
Laporte, J ;
Blondeau, F ;
Buj-Bello, A ;
Mandel, JL .
TRENDS IN GENETICS, 2001, 17 (04) :221-228
[28]  
MANDEL JL, 2002, STRUCTURAL MOL BASIS, P124
[29]   Gene targeting restricted to mouse striated muscle lineage [J].
Miniou, Pierre ;
Tiziano, Danilo ;
Frugier, Tony ;
Roblot, Natacha ;
Le Meur, Marianne ;
Melki, Judith .
NUCLEIC ACIDS RESEARCH, 1999, 27 (19) :e27
[30]   Characterization of an adapter subunit to a phosphatidylinositol (3)P 3-phosphatase: Identification of a myotubularin-related protein lacking catalytic activity [J].
Nandurkar, HH ;
Caldwell, KK ;
Whisstock, JC ;
Layton, MJ ;
Gaudet, EA ;
Norris, FA ;
Majerus, PW ;
Mitchell, CA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (17) :9499-9504