Electrokinetic focusing and filtration of cells in a serpentine microchannel

被引：69

作者：

Church, Christopher ^{[1
]}

Zhu, Junjie ^{[1
]}

Wang, Gaoyan ^{[2
]}

Tzeng, Tzuen-Rong J. ^{[2
]}

Xuan, Xiangchun ^{[1
]}

机构：

[1] Clemson Univ, Dept Mech Engn, Clemson, SC 29634 USA

[2] Clemson Univ, Dept Biol Sci, Clemson, SC 29634 USA

来源：

BIOMICROFLUIDICS | 2009年 / 3卷 / 04期

基金：

美国国家科学基金会;

关键词：

biochemistry; bioelectric phenomena; biological effects of fields; cellular effects of radiation; electrokinetic effects; electrophoresis; microchannel flow; DIRECT-CURRENT-DIELECTROPHORESIS; MICROFLUIDIC DEVICES; ELECTROPHORETIC MOTION; FLOW-CYTOMETRY; PARTICLES; SEPARATION; FORCES; MANIPULATION; CHANNEL; FIELDS;

D O I：

10.1063/1.3267098

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Focusing cells into a single stream is usually a necessary step prior to counting and separating them in microfluidic devices such as flow cytometers and cell sorters. This work presents a sheathless electrokinetic focusing of yeast cells in a planar serpentine microchannel using dc-biased ac electric fields. The concurrent pumping and focusing of yeast cells arise from the dc electrokinetic transport and the turn-induced ac/dc dielectrophoretic motion, respectively. The effects of electric field (including ac to dc field ratio and ac field frequency) and concentration (including buffer concentration and cell concentration) on the cell focusing performance were studied experimentally and numerically. A continuous electrokinetic filtration of E. coli cells from yeast cells was also demonstrated via their differential electrokinetic focusing in a serpentine microchannel.

引用

页数：10

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