Towards the total chemical synthesis of integral membrane proteins:: a general method for the synthesis of hydrophobic peptide-αthioester building blocks

被引：79

作者：

Johnson, Erik C. B.

Kent, Stephen B. H.

机构：

[1] Inst Biophys Dynam, Chicago, IL 60637 USA

[2] Univ Chicago, Dept Biochem, Chicago, IL 60637 USA

[3] Univ Chicago, Dept Chem, Chicago, IL 60637 USA

来源：

TETRAHEDRON LETTERS | 2007年 / 48卷 / 10期

关键词：

chemical protein synthesis; integral membrane proteins; diacylglycerol kinase; native chemical ligation; thioester;

D O I：

10.1016/j.tetlet.2007.01.030

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Modification of a peptide-(alpha)thioester with a sequence of six arginines on the thioester leaving group can render soluble all peptides derived from a polytopic integral membrane protein. This strategy greatly simplifies the synthesis of peptide-(alpha)thioester building blocks for the total chemical synthesis of integral membrane proteins by native chemical ligation. (c) 2007 Elsevier Ltd. All rights reserved.

引用

收藏

页码：1795 / 1799

页数：5

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