Multiple Soft Ionization of Gas-Phase Proteins and Swift Backbone Dissociation in Collisions with ≤ 99 eV Electrons

被引：15

作者：

Zubarev, Roman A. ^{[1
]}

Yang, Hongqian ^{[1
]}

机构：

[1] Karolinska Inst, Div Mol Biometry, Inst Med Biochem & Biophys, S-17177 Stockholm, Sweden

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 08期

基金：

瑞典研究理事会;

关键词：

electron capture dissociation; electron impact dissociation; mass spectrometry; proteins; proteomics; TANDEM MASS-SPECTROMETRY; TRIPLE IONIZATION; CAPTURE DISSOCIATION; MOLECULES; CATIONS; IMPACT;

D O I：

10.1002/anie.200905977

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Contrary to the dominant paradigm, electrons with an energy above 20 eV can ionize polypeptides multiple times (see picture) without destroying their primary or even elements of their tertiary structure. Concomitant electronic excitation can cause rapid backbone-bond cleavage, with weak molecule-molecule bonding remaining intact. This"soft- fragmentation" technique should enable the detailed structural characterization of protein complexes. © 2010 Wiley-VCH Verlag GmbH &. Co. KGaA,.

引用

页码：1439 / 1441

页数：3

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