Surface Modification of Upconverting NaYF4 Nanoparticles with PEG-Phosphate Ligands for NIR (800 nm) Biolabeling within the Biological Window

被引：396

作者：

Boyer, John-Christopher ^{[1
]}

Manseau, Marie-Pascale ^{[1
]}

Murray, Jill I. ^{[1
]}

van Veggel, Frank C. J. M. ^{[1
]}

机构：

[1] Univ Victoria, Dept Chem, Victoria, BC V8W 3V6, Canada

来源：

LANGMUIR | 2010年 / 26卷 / 02期

基金：

加拿大创新基金会; 加拿大自然科学与工程研究理事会;

关键词：

MULTICOLOR UP-CONVERSION; QUANTUM DOTS; NANOCRYSTALS; LUMINESCENCE; CORE; CELLS; YB3+;

D O I：

10.1021/la902260j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We present a technique for the replacement of oleate with a PEG-phosphate ligand [PEG = poly(ethylene glycol)] as an efficient method for the generation of water-dispersible NaYF4 nanoparticles (NPs). The PEG-phosphate ligands are shown to exchange with the original oleate ligands on the Surface of the NPs, resulting in water-dispersible NPs. The upconversion intensity of the NPs in aqueous environments was found to be severely quenched when compared to the original NPs in organic solvents. This is attributed to an increase in the multiphotion relaxations of the lanthanide excited state in aqueous environments due to high energy vibrational modes of water molecules. This problem could be overcome partially by the synthesis of core/shell NPs which demonstrated improved photophysical properties in water over the original core NPs. The PEG-phosphate coated upconverting NPs were then used to image a line of ovarian cancer cells (CaOV3) to demonstrate their promise biological application.

引用

页码：1157 / 1164

页数：8

共 36 条

[11] Biocompatibility of silica coated NaYF4 upconversion fluorescent nanocrystals [J].

Jalil, Rufaihah Abdul ;

Zhang, Yong .

BIOMATERIALS, 2008, 29 (30) :4122-4128

[12] NIR-to-visible upconversion nanoparticles for fluorescent labeling and targeted delivery of siRNA [J].

Jiang, Shan ;

Zhang, Yong ;

Lim, Kian Meng ;

Sim, Eugene K. W. ;

Ye, Lei .

NANOTECHNOLOGY, 2009, 20 (15)

[13] Combined Optical and MR Bioimaging Using Rare Earth Ion Doped NaYF4 Nanocrystals [J].

Kumar, Rajiv ;

Nyk, Marcin ;

Ohulchanskyy, Tymish Y. ;

Flask, Christopher A. ;

Prasad, Paras N. .

ADVANCED FUNCTIONAL MATERIALS, 2009, 19 (06) :853-859

[14] PEG-ylated cationic CdSe/ZnS QDs as an efficient intracellular labeling agent [J].

Lee, Junghan ;

Kim, Junwon ;

Park, Eunjung ;

Jo, Shineun ;

Song, Rita .

PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 2008, 10 (13) :1739-1742

[15]

LI Z, 2008, NANOTECHNOLOGY, V19, P1

[16] Multicolor Core/Shell-Structured Upconversion Fluorescent Nanoparticles [J].

Li, Zhengquan ;

Zhang, Yong ;

Jiang, Shan .

ADVANCED MATERIALS, 2008, 20 (24) :4765-+

[17]

LIU Y, 2008, J APPL PHYS, V103

[18] Highly efficient multicolor up-conversion emissions and their mechanisms of monodisperse NaYF4:Yb,Er core and core/shell-structured nanocrystals [J].

Mai, Hao-Xin ;

Zhang, Ya-Wen ;

Sun, Ling-Dong ;

Yan, Chun-Hua .

JOURNAL OF PHYSICAL CHEMISTRY C, 2007, 111 (37) :13721-13729

[19] High-quality sodium rare-earth fluoride nanocrystals: Controlled synthesis and optical properties [J].

Mai, Hao-Xin ;

Zhang, Ya-Wen ;

Si, Rui ;

Yan, Zheng-Guang ;

Sun, Ling-Dong ;

You, Li-Ping ;

Yan, Chun-Hua .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (19) :6426-6436

[20] Characterization of synthetic polymers by MALDI-TOF/MS: Investigation into new methods of sample target preparation and consequence on mass spectrum finger print [J].

Marie, A ;

Fournier, F ;

Tabet, JC .

ANALYTICAL CHEMISTRY, 2000, 72 (20) :5106-5114

← 1 2 3 4 →