Suppressive effect of Clostridium Perfringens-produced heat-stable substance(s) on proliferation of human colon adenocarcinoma HT29 cells in culture

被引：12

作者：

Arimochi, Hideki ^{[1
]}

Morita, Kyoji

Kataoka, Keiko

Nakanishi, Shusuke

Kuwahara, Tomomi

Ohnishi, Yoshinari

机构：

[1] Univ Tokushima, Grad Sch Med, Dept Mol Bacteriol, Tokushima 7708503, Japan

[2] Univ Tokushima, Sch Med, Dept Pharmacol, Tokushima 7708503, Japan

[3] Miyarisan Pharmaceut Co, Res Lab, Nagano 3890602, Japan

来源：

CANCER LETTERS | 2006年 / 241卷 / 02期

关键词：

Clostridium perfringens; bacterial culture medium; cytostatic effect; egr-1; expression; human adenocarcinoma HT29 cell; colon cancer;

D O I：

10.1016/j.canlet.2005.10.016

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Clostridium perfringens has been regarded as one of the intestinal bacteria increasing colon cancer risk. In previous studies, we have shown that the oral administration of C. perfringens culture medium can inhibit the mutagen-induced formation of pre-neoplastic lesions in rat colon, thus proposing the existence of factor(s) preventing colon tumorigenesis in this culture medium. However, the properties of effective factor(s) and the mechanism of this inhibitory action still remain to be investigated. Then, the effect of C. perfringens culture medium on human colon adenocarcinoma HT29 cells was examined. The exposure of HT29 cells to C. perfringens culture medium was found to suppress the proliferation of these cells probably through the reduction of immediate early gene egr-1 expression. These observations suggest that C. perfringens culture medium has a cytostatic action on colon tumor cells, which may be responsible for the prevention of pre-neoplastic formation in rat colon. (c) 2006 Elsevier Ireland Ltd. All rights reserved.

引用

页码：228 / 234

页数：7

共 24 条

[1] Effect of intestinal bacteria on formation of azoxymethane-induced aberrant crypt foci in the rat colon [J].

Arimochi, H ;

Kinouchi, T ;

Kataoka, K ;

Kuwahara, T ;

Ohnishi, Y .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1997, 238 (03) :753-757

[2] BDNF and trkB mRNAs oscillate in rat brain during the light-dark cycle [J].

Bova, R ;

Micheli, MR ;

Qualadrucci, P ;

Zucconi, GG .

MOLECULAR BRAIN RESEARCH, 1998, 57 (02) :321-324

[3]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[4]

Cato E.P., 1986, Bergey's Manual of Systematic Bacteriology, V2, P1141

[5]

CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2

[6] APPLICATION OF THE NEUTRAL RED ASSAY (NR ASSAY) TO MONOLAYER-CULTURES OF PRIMARY HEPATOCYTES - RAPID COLORIMETRIC VIABILITY DETERMINATION FOR THE UNSCHEDULED DNA-SYNTHESIS TEST (UDS) [J].

FAUTZ, R ;

HUSEIN, B ;

HECHENBERGER, C .

MUTATION RESEARCH, 1991, 253 (02) :173-179

[7] c-jun and Egr-1 participate in DNA synthesis and cell survival in response to ionizing radiation exposure [J].

Hallahan, DE ;

Dunphy, E ;

Virudachalam, S ;

Sukhatme, VP ;

Kufe, DW ;

Weichselbaum, RR .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (51) :30303-30309

[8] The effects of short-chain fatty acids on human colon cancer cell phenotype are associated with histone hyperacetylation [J].

Hinnebusch, BF ;

Meng, SF ;

Wu, JT ;

Archer, SY ;

Hodin, RA .

JOURNAL OF NUTRITION, 2002, 132 (05) :1012-1017

[9] Intestinal bacterial β-glucuronidase activity of patients with colon cancer [J].

Kim, DH ;

Jin, YH .

ARCHIVES OF PHARMACAL RESEARCH, 2001, 24 (06) :564-567

[10] Clostridium hiranonis sp nov., a human intestinal bacterium with bile acid 7α-dehydroxylating activity [J].

Kitahara, M ;

Takamine, F ;

Imamura, T ;

Benno, Y .

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2001, 51 :39-44

← 1 2 3 →