Actomyosin meshwork mechanosensing enables tissue shape to orient cell force

被引:98
作者
Chanet, Soline [1 ]
Miller, Callie J. [2 ]
Vaishnav, Eeshit Dhaval [1 ]
Ermentrout, Bard [3 ]
Davidson, Lance A. [2 ,4 ,5 ]
Martin, Adam C. [1 ]
机构
[1] MIT, Dept Biol, 77 Massachusetts Ave, Cambridge, MA 02142 USA
[2] Univ Pittsburgh, Dept Bioengn, Pittsburgh, PA 15260 USA
[3] Univ Pittsburgh, Dept Math, Pittsburgh, PA 15260 USA
[4] Univ Pittsburgh, Dept Dev Biol, Pittsburgh, PA 15260 USA
[5] Univ Pittsburgh, Dept Computat & Syst Biol, Pittsburgh, PA 15260 USA
基金
美国国家卫生研究院; 美国国家科学基金会;
关键词
DRIVE APICAL CONSTRICTION; ACTIN NETWORK; EPITHELIAL MORPHOGENESIS; DROSOPHILA GASTRULATION; PULSED CONTRACTIONS; PLANAR POLARITY; AXIS ELONGATION; ADHERENT CELLS; MOTOR-ACTIVITY; MYOSIN-II;
D O I
10.1038/ncomms15014
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];
摘要
Sculpting organism shape requires that cells produce forces with proper directionality. Thus, it is critical to understand how cells orient the cytoskeleton to produce forces that deform tissues. During Drosophila gastrulation, actomyosin contraction in ventral cells generates a long, narrow epithelial furrow, termed the ventral furrow, in which actomyosin fibres and tension are directed along the length of the furrow. Using a combination of genetic and mechanical perturbations that alter tissue shape, we demonstrate that geometrical and mechanical constraints act as cues to orient the cytoskeleton and tension during ventral furrow formation. We developed an in silico model of two-dimensional actomyosin meshwork contraction, demonstrating that actomyosin meshworks exhibit an inherent force orienting mechanism in response to mechanical constraints. Together, our in vivo and in silico data provide a framework for understanding how cells orient force generation, establishing a role for geometrical and mechanical patterning of force production in tissues.
引用
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页数:13
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