Controlling the selection stringency of phage display using a microfluidic device

被引：51

作者：

Liu, Yanli ^{[1
,2
]}

Adams, Jonathan D. ^{[3
]}

Turner, Kelisha ^{[5
]}

Cochran, Frank V. ^{[6
,7
]}

Gambhir, Sanjiv Sam ^{[6
,7
]}

Soh, H. Tom ^{[1
,4
]}

机构：

[1] Univ Calif Santa Barbara, Dept Mat, Santa Barbara, CA 93106 USA

[2] Univ Calif Santa Barbara, Neurosci Res Inst, Santa Barbara, CA 93106 USA

[3] Univ Calif Santa Barbara, Dept Phys, Santa Barbara, CA 93106 USA

[4] Univ Calif Santa Barbara, Dept Mech Engn, Santa Barbara, CA 93106 USA

[5] Jackson State Univ, Sch Sci & Technol, Jackson, MS 39217 USA

[6] Stanford Univ, Mol Imaging Program Stanford, Dept Radiol, Stanford, CA 94305 USA

[7] Stanford Univ, Bio X Program, Stanford, CA 94305 USA

来源：

LAB ON A CHIP | 2009年 / 9卷 / 08期

基金：

美国国家卫生研究院;

关键词：

PEPTIDE LIBRARIES; AFFINITY LIGANDS; SEPARATION; DISCOVERY; APTAMERS; SURFACE; PHASE; MODEL; CHIP; DRUG;

D O I：

10.1039/b820985e

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the utilization of microfluidic technology to phage selection and demonstrate that accurate control of washing stringency in our microfluidic magnetic separator (MMS) directly impacts the diversity of isolated peptide sequences. Reproducible generation of magnetic and fluidic forces allows controlled washing conditions that enable rapid convergence of selected peptide sequences. These findings may provide a foundation for the development of automated microsystems for rapid in vitro directed evolution of affinity reagents.

引用

页码：1033 / 1036

页数：4

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