In vivo trafficking and targeted delivery of magnetically labeled stem cells

被引：153

作者：

Arbab, AS ^{[1
]}

Jordan, EK ^{[1
]}

Wilson, LB ^{[1
]}

Yocum, GT ^{[1
]}

Lewis, BK ^{[1
]}

Frank, JA ^{[1
]}

机构：

[1] NIH, Expt Neuroimaging Sect, Lab Diagnost Radiol Res, Bethesda, MD 20892 USA

来源：

HUMAN GENE THERAPY | 2004年 / 15卷 / 04期

关键词：

D O I：

10.1089/104303404322959506

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

Targeted delivery of intravenously administered genetically altered cells or stem cells is still in an early stage of investigation. We developed a method of delivering iron oxide (ferumoxide)-labeled mesenchymal stem cells (MSCs) to a targeted area in an animal model by applying an external magnet. Rats with or without an external magnet placed over the liver were injected intravenously with ferumoxide-labeled MSCs and magnetic resonance imaging (MRI) signal intensity (SI) changes, iron concentration, and concentration of MSCs in the liver were monitored at different time points. SI decreased in the liver after injection of MSCs and returned gradually to that of control rat livers at approximately day 29. SI decreases were greater in rats with external magnets. Higher iron concentration and increased labeled cell numbers were detected in rat livers with external magnets. The external magnets influenced the movement of labeled MSCs such that the cells were retained in the region of interest. These results potentially open a new area of investigation for delivering stem cells or genetically altered cells.

引用

页码：351 / 360

页数：10

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