Strong resistance of a thin crystalline layer of balanced charged groups to protein adsorption

被引:205
作者
Chen, Shengfu [1 ]
Yu, Fuchen [1 ]
Yu, Qiuming [1 ]
He, Yi [1 ]
Jiang, Shaoyi [1 ]
机构
[1] Univ Washington, Dept Chem Engn, Seattle, WA 98195 USA
关键词
D O I
10.1021/la061012m
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Resistance of mixed self-assembled monolayers (SAMs) with various counter-charged terminal groups of different valence and protonation/ deprotonation states to nonspecific protein adsorption is investigated. It is demonstrated that excellent nonfouling surfaces can be readily constructed from mixed positively and negatively charged components of equal valence in a wide range of thiol solution compositions. Furthermore, the lattice structure of one of the mixed SAM systems studied is revealed by atomic force microscopy (AFM) to be (5.2 +/- 0.2 angstrom +/- 5.2 (0.2 angstrom)60 degrees. Results indicate that the packing structure of mixed charged SAMs is determined by strong charge-charge interactions of the terminal groups rather than S-Au and chain-chain interactions. This work provides direct evidence that conformational flexibility is not required for protein resistance of a surface and even a single compact layer of charged groups of balanced charge with a crystalline structure can resist nonspecific protein adsorption, suggesting that tightly bound water molecules on the topmost part of the mixed SAMs play a dominant role in surface resistance to nonspecific protein adsorption.
引用
收藏
页码:8186 / 8191
页数:6
相关论文
共 28 条
[1]   INFRARED-ABSORPTION AND RAMAN-SCATTERING OF SULFATE GROUPS OF HEPARIN AND RELATED GLYCOSAMINOGLYCANS IN AQUEOUS-SOLUTION [J].
CABASSI, F ;
CASU, B ;
PERLIN, AS .
CARBOHYDRATE RESEARCH, 1978, 63 (JUN) :1-11
[2]   Strong resistance of phosphorylcholine self-assembled monolayers to protein adsorption: Insights into nonfouling properties of zwitterionic materials [J].
Chen, SF ;
Zheng, J ;
Li, LY ;
Jiang, SY .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (41) :14473-14478
[3]   Strong resistance of oligo(phosphorylcholine) self-assembled monolayers to protein adsorption [J].
Chen, SF ;
Liu, LY ;
Jiang, SY .
LANGMUIR, 2006, 22 (06) :2418-2421
[4]   Adsorption of fibrinogen and lysozyme on silicon grafted with poly(2-methacryloyloxyethyl phosphorylcholine) via surface-initiated atom transfer radical polymerization [J].
Feng, W ;
Zhu, SP ;
Ishihara, K ;
Brash, JL .
LANGMUIR, 2005, 21 (13) :5980-5987
[5]  
GLASMASTER KC, 2001, J COLLOID INTERF SCI, V264, P40
[6]  
Harris J. M., 1992, POLY ETHYLENE GLYCOL
[7]   Zwitterionic SAMs that resist nonspecific adsorption of protein from aqueous buffer [J].
Holmlin, RE ;
Chen, XX ;
Chapman, RG ;
Takayama, S ;
Whitesides, GM .
LANGMUIR, 2001, 17 (09) :2841-2850
[8]   Spectral surface plasmon resonance biosensor for detection of staphylococcal enterotoxin B in milk [J].
Homola, J ;
Dostálek, J ;
Chen, SF ;
Rasooly, A ;
Jiang, SY ;
Yee, SS .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2002, 75 (1-2) :61-69
[9]   Modification of polysulfone with phospholipid polymer for improvement of the blood compatibility. Part 1. Surface characterization [J].
Ishihara, K ;
Fukumoto, K ;
Iwasaki, Y ;
Nakabayashi, N .
BIOMATERIALS, 1999, 20 (17) :1545-1551
[10]   PROTEIN SURFACE INTERACTIONS IN THE PRESENCE OF POLYETHYLENE OXIDE .1. SIMPLIFIED THEORY [J].
JEON, SI ;
LEE, JH ;
ANDRADE, JD ;
DEGENNES, PG .
JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1991, 142 (01) :149-158