Technical problems and practical operations in plant metabolomics

被引：8

作者：

Bamba, Takeshi

Fukusaki, Eiichiro

机构：

[1] Osaka Univ, Dept Biotechnol, Grad Sch Engn, Suita, Osaka 5650871, Japan

[2] Osaka Univ, Grad Sch Pharmaceut Sci, Dept Appl Environm Biol, Suita, Osaka 5650871, Japan

来源：

JOURNAL OF PESTICIDE SCIENCE | 2006年 / 31卷 / 03期

关键词：

metabolomics; metabolome; metabolite profiling; metabolic fingerprinting; mass spectrometry; chemometrics;

D O I：

10.1584/jpestics.31.300

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

A metabolomics experiment consists of several complicated technical elements with each step ('cultivation of organisms,' `sampling,' 'sample preparation,' 'analysis,' 'data conversion,' and 'informatics') potentially giving rise to experimental error. In order to perform metabolomics studies, it is necessary to understand the method limitations in detail and recognize possible problems at each step. Here, we review a number of technical problems associated with plant metabolomics and describe some practical knowledge for experimental design. (c) Pesticide Science Society of Japan.

引用

页码：300 / 304

页数：5

共 46 条

[41] Analysis of highly polar compounds of plant origin: Combination of hydrophilic interaction chromatography and electrospray ion trap mass spectrometry [J].