Technical problems and practical operations in plant metabolomics

被引：8

作者：

Bamba, Takeshi

Fukusaki, Eiichiro

机构：

[1] Osaka Univ, Dept Biotechnol, Grad Sch Engn, Suita, Osaka 5650871, Japan

[2] Osaka Univ, Grad Sch Pharmaceut Sci, Dept Appl Environm Biol, Suita, Osaka 5650871, Japan

来源：

JOURNAL OF PESTICIDE SCIENCE | 2006年 / 31卷 / 03期

关键词：

metabolomics; metabolome; metabolite profiling; metabolic fingerprinting; mass spectrometry; chemometrics;

D O I：

10.1584/jpestics.31.300

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

A metabolomics experiment consists of several complicated technical elements with each step ('cultivation of organisms,' `sampling,' 'sample preparation,' 'analysis,' 'data conversion,' and 'informatics') potentially giving rise to experimental error. In order to perform metabolomics studies, it is necessary to understand the method limitations in detail and recognize possible problems at each step. Here, we review a number of technical problems associated with plant metabolomics and describe some practical knowledge for experimental design. (c) Pesticide Science Society of Japan.

引用

收藏

页码：300 / 304

页数：5

相关论文

共 46 条

[21] Metabolic fingerprinting of salt-stressed tomatoes [J].

Johnson, HE ;

Broadhurst, D ;

Goodacre, R ;

Smith, AR .

PHYTOCHEMISTRY, 2003, 62 (06) :919-928

[22] A strategy for identifying differences in large series of metabolomic samples analyzed by GC/MS [J].

Jonsson, P ;

Gullberg, J ;

Nordström, A ;

Kusano, M ;

Kowalczyk, M ;

Sjöström, M ;

Moritz, T .

ANALYTICAL CHEMISTRY, 2004, 76 (06) :1738-1745

[23] High-throughput data analysis for detecting and identifying differences between samples in GC/MS-based metabolomic analyses [J].

Jonsson, P ;

Johansson, AI ;

Gullberg, J ;

Trygg, J ;

A, J ;

Grung, B ;

Marklund, S ;

Sjoström, M ;

Antti, H ;

Moritz, T .

ANALYTICAL CHEMISTRY, 2005, 77 (17) :5635-5642

[24] Predictive metabolite profiling applying hierarchical multivariate curve resolution to GC-MS datas -: A potential tool for multi-parametric diagnosis [J].

Jonsson, Par ;

Johansson, Elin Sjovik ;

Wuolikainen, Anna ;

Lindberg, Johan ;

Schuppe-Koistinen, Ina ;

Kusano, Miyako ;

Sjostrom, Michael ;

Trygg, Johan ;

Moritz, Thomas ;

Antti, Henrik .

JOURNAL OF PROTEOME RESEARCH, 2006, 5 (06) :1407-1414

[25] Stable isotope dilution-based accurate comparative quantification of nitrogen-containing metabolites in Arabidopsis thaliana T87 cells using in vivo 15N-isotope enrichment [J].

Kim, JK ;

Harada, K ;

Bamba, T ;

Fukusaki, E ;

Kobayashi, A .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2005, 69 (07) :1331-1340

[26] Mechanistic investigation of ionization suppression in electrospray ionization [J].

King, R ;

Bonfiglio, R ;

Fernandez-Metzler, C ;

Miller-Stein, C ;

Olah, T .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2000, 11 (11) :942-950

[27] Lactofen induces isoflavone accumulation and glyceollin elicitation competency in soybean [J].

Landini, S ;

Graham, MY ;

Graham, TL .

PHYTOCHEMISTRY, 2003, 62 (06) :865-874

[28] Analysis of free amino acids in fermented shrimp waste by high-performance liquid chromatography [J].

López-Cervantes, J ;

Sánchez-Machado, DI ;

Rosas-Rodríguez, JA .

JOURNAL OF CHROMATOGRAPHY A, 2006, 1105 (1-2) :106-110

[29] Ion suppression effects in liquid chromatography-electrospray-ionisation transport-region collision induced dissociation mass spectrometry with different serum extraction methods for systematic toxicological analysis with mass spectra libraries [J].

Müller, C ;

Schäfer, P ;

Störtzel, M ;

Vogt, S ;

Weinmann, W .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2002, 773 (01) :47-52

[30] Nonlinear data alignment for UPLC-MS and HPLC-MS based metabolomics:: Quantitative analysis of endogenous and exogenous metabolites in human serum [J].

Nordstrom, Anders ;

O'Maille, Grace ;

Qin, Chuan ;

Siuzdak, Gary .

ANALYTICAL CHEMISTRY, 2006, 78 (10) :3289-3295

← 1 2 3 4 5 →