Thin-layer chromatography combined with diode laser desorption/atmospheric pressure chemical ionization mass spectrometry

被引：27

作者：

Peng, S ^{[1
]}

Ahlmann, N ^{[1
]}

Kunze, K ^{[1
]}

Nigge, W ^{[1
]}

Edler, M ^{[1
]}

Hoffmann, T ^{[1
]}

Franzke, J ^{[1
]}

机构：

[1] ISAS, Inst Analyt Sci, D-44139 Dortmund, Germany

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 2004年 / 18卷 / 16期

关键词：

D O I：

10.1002/rcm.1556

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The desorption of an analyte by a continuous wave diode laser from a porous surface of a thin-layer plate covered with a graphite suspension is presented. The thermally desorbed analyte molecules are ionized in the gas phase by a corona discharge at atmospheric pressure. Therefore, both essential processes-the desorption and the ionization of analyte molecules, which are often performed in one step-are separated. The target preparation is easy and fast since no additional extraction process is required. The mass spectrometric background signal was mostly limited to the low mass range showing no interference with typical compounds of interest. In this study, the calmative and antihypertensive drug reserpine was chosen as model analyte, which is often used for specification of mass spectrometers. No fragmentation was observed because of efficient collisional cooling under atmospheric pressure. The influence of diode laser power and the composition of the graphite suspension were investigated, and a primary optimization was performed. Copyright (C) 2004 John Wiley Sons, Ltd.

引用

页码：1803 / 1808

页数：6

共 25 条

[1] Thin-layer chromatography mass spectrometry using activated carbon, surface-assisted laser desorption/ionization [J].