Coumarin-fluorescein pair as a new donor-acceptor set for fluorescence energy transfer study of DNA

被引：17

作者：

Mitsui, T ^{[1
]}

Nakano, H ^{[1
]}

Yamana, K ^{[1
]}

机构：

[1] Himeji Inst Technol, Dept Appl Chem, Himeji, Hyogo 6712201, Japan

来源：

TETRAHEDRON LETTERS | 2000年 / 41卷 / 15期

关键词：

coumarin-fluorescein pair; nucleoside; fluorescence energy transfer; DNA;

D O I：

10.1016/S0040-4039(00)00215-X

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A method for introduction of the 2'-coumarin labeled nucleoside as a fluorescence energy donor into DNA duplexes has been described. Efficient FRET occurs between the coumarin-fluorescein pair in DNA owing to the high quantum yield of the donor. The present donor-acceptor pair may be useful as FRET indicator of DNA structures in solution, (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：2605 / 2608

页数：4

共 11 条

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