A NOVEL MICROFLUIDIC PLATFORM FOR CONTINUOUS DNA EXTRACTION AND PURIFICATION USING LAMINAR FLOW MAGNETOPHORESIS

被引：12

作者：

Karle, Marc ^{[1
]}

Miwa, Junichi ^{[2
]}

Roth, Guenter ^{[2
]}

Zengerle, Roland ^{[1
,2
]}

von Stetten, Felix ^{[1
,2
]}

机构：

[1] HSG IMIT, Villingen Schwenningen, Germany

[2] Univ Freiburg, IMTEK, Freiburg, Germany

来源：

IEEE 22ND INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS 2009) | 2009年

关键词：

CHIP; PCR;

D O I：

10.1109/MEMSYS.2009.4805372

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

We present a novel microfluidic platform using laminar-flow magnetophoresis for combined continuous extraction and purification of DNA. All essential unit operations (DNA binding, sample washing and DNA elution) are integrated on one single chip. The key function is the motion of magnetic beads given by the interplay of laminar flow and time-varying magnetic field. The time for extraction was 1 minute. The device is a central part of a complete biochemical system for continuous monitoring of cell-growth in bioreactors. The novel platform allows continuous purification of DNA, but is also applicable to purification of RNA, proteins or cells, including their subsequent real-time analysis in general.

引用

页码：276 / 279

页数：4

共 9 条

[1] Continuous molecular enrichment in microfluidic systems [J].