Prions Are Secreted in Milk from Clinically Normal Scrapie-Exposed Sheep

被引：39

作者：

Maddison, B. C. ^{[2
]}

Baker, C. A. ^{[2
]}

Rees, H. C.

Terry, L. A. ^{[3
]}

Thorne, L. ^{[3
]}

Bellworthy, S. J. ^{[3
]}

Whitelam, G. C.

Gough, K. C. ^{[1
]}

机构：

[1] Univ Nottingham, Sch Vet Med & Sci, Loughborough LE12 5RD, Leics, England

[2] Univ Leicester, Dept Biol, ADAS UK, Leicester LE1 7RH, Leics, England

[3] Vet Labs Agcy, Addlestone KT15 3NB, Surrey, England

来源：

JOURNAL OF VIROLOGY | 2009年 / 83卷 / 16期

关键词：

CREUTZFELDT-JAKOB-DISEASE; CHRONIC WASTING DISEASE; IN-VITRO AMPLIFICATION; BOVINE SPONGIFORM ENCEPHALOPATHY; CYCLIC AMPLIFICATION; INFECTIOUS PRIONS; PRESYMPTOMATIC DETECTION; PROTEIN; BLOOD; TRANSMISSION;

D O I：

10.1128/JVI.00051-09

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The potential spread of prion infectivity in secreta is a crucial concern for prion disease transmission. Here, serial protein misfolding cyclic amplification (sPMCA) allowed the detection of prions in milk from clinically affected animals as well as scrapie-exposed sheep at least 20 months before clinical onset of disease, irrespective of the immunohistochemical detection of protease-resistant PrPSc within lymphoreticular and central nervous system tissues. These data indicate the secretion of prions within milk during the early stages of disease progression and a role for milk in prion transmission. Furthermore, the application of sPMCA to milk samples offers a noninvasive methodology to detect scrapie during preclinical/subclinical disease.

引用

页码：8293 / 8296

页数：4

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