HPLC and Chemometrics for the Quality Consistency Evaluation of Shuanghuanglian Injection

被引：16

作者：

Luan, Lan ^{[1
,2
]}

Wang, Gangli ^{[2
]}

Lin, Ruichao ^{[2
]}

机构：

[1] China Acad Tradit Chinese Med, China Acad Chinese Med Sci, Beijing 100700, Peoples R China

[2] Natl Inst Food & Drug Control, Beijing 100050, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHIC SCIENCE | 2014年 / 52卷 / 07期

关键词：

FORSYTHIA-SUSPENSA VAHL; IN-VITRO; CHINESE; SPECTROMETRY; FINGERPRINT; MEDICINE; VIRUS; ACID; RATS;

D O I：

10.1093/chromsci/bmt104

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

A high-performance liquid chromatography method with diode array detection was developed for simultaneous analysis of organic acids (chlorogenic acid, caffeic acid, 4,5-dicaffeoylquinic acid) and flavonoids (baicalin, baicalein, wogonin) in Shuang-huang-lian injection, a traditional Chinese medicine being prescribed widely for antibacterial, anti-viral and immunostimulant activity. All calibration curves showed good linear correlation (r(2) > 0.9997) within the test ranges. Validation proved that the repeatability of the method was good and recovery was satisfactory. The validated method was successfully applied to 24 batches of Shuang-huang-lian injection. The result showed that there was a great variation among different samples. Principal component analysis further proved considerable variations among the samples from eight manufacturers, and suggested that chlorogenic acid and baicalin might have the greatest variations among the 24 batches. In conclusion, these results demonstrated that the method proposed was very useful for the analysis and quality consistency evaluation of Shuang-huang-lian injection.

引用

页码：707 / 712

页数：6

共 28 条

[11]

Luo Qi-zhi, 2009, Yaoxue Xuebao, V44, P1391

[12]

Antiviral Chinese medicinal herbs against respiratory syncytial virus [J].

Ma, SC ;

Du, J ;

But, PPH ;

Deng, XL ;

Zhang, YW ;

Ooi, VEC ;

Xu, HX ;

Lee, SHS ;

Lee, SF .

JOURNAL OF ETHNOPHARMACOLOGY, 2002, 79 (02) :205-211

[13]

Antiviral amentoflavone from Selaginella sinensis [J].

Ma, SC ;

But, PPH ;

Ooi, VEC ;

He, YH ;

Lee, SHS ;

Lee, SF ;

Lin, RC .

BIOLOGICAL & PHARMACEUTICAL BULLETIN, 2001, 24 (03) :311-312

[14]

In vitro evaluation of secoiridoid glucosides from the fruits of Ligustrum lucidum as antiviral agents [J].

Ma, SC ;

He, ZD ;

Deng, XL ;

But, PPH ;

Ooi, VEC ;

Xu, HX ;

Lee, SHS ;

Lee, SF .

CHEMICAL & PHARMACEUTICAL BULLETIN, 2001, 49 (11) :1471-1473

[15]

Ozaki Y, 1997, BIOL PHARM BULL, V20, P861, DOI 10.1248/bpb.20.861

[16]

Ozaki Y, 2000, BIOL PHARM BULL, V23, P365, DOI 10.1248/bpb.23.365

[17]

[彭维 Peng Wei], 2010, [中山大学学报. 自然科学版, Acta Scientiarum Naturalium Universitatis Sunyatseni], V49, P142

[18]

Qiu Qiyuan, 2012, PHARM CLIN RES, V20, P402

[19]

A strategy for the detection of quality fluctuation of a Chinese herbal injection based on chemical fingerprinting combined with biological fingerprinting [J].

Ren, Yongshen ;

Zhang, Ping ;

Yan, Dan ;

Wang, Jiabo ;

Du, Xiaoxi ;

Xiao, Xiaohe .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2011, 56 (02) :436-442

[20]

The State Pharmacopoeia Commission of PR China, 2010, PHARM PEOPL REP CHIN, V1, P846

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