Leptin expression in breast nipple aspirate fluid (NAF) and serum is influenced by body mass index (BMI) but not by the presence of breast cancer

被引:40
作者
Sauter, ER
Garofalo, C
Hewett, J
Hewett, JE
Morelli, C
Surmacz, E
机构
[1] Univ Missouri, Ellis Fischel Canc Ctr, Columbia, MO 65212 USA
[2] Thomas Jefferson Univ, Kimmel Canc Ctr, Philadelphia, PA USA
关键词
obesity; premenopause; postmenopause; protein concentration; leptin release;
D O I
10.1055/s-2004-814490
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
While obesity is a known risk factor for postmenopausal breast cancer, the molecular mechanisms involved are unclear. Systemic levels of leptin, the product of the ob (obesity) gene, are increased in obese individuals (body mass index, BMI, over 25) and are higher in women than men. Leptin has been found to stimulate the growth of breast cancer cells in vitro. Our goal was to determine whether leptin was 1) present in nipple aspirate fluid (NAF), and 2) whether NAF leptin levels were associated with a) levels in serum, b) obesity, and c) breast cancer. We collected and evaluated NAF specimens from 83 subjects and serum specimens from 49 subjects. NAF leptin was detectable in 16/41 (39%) of premenopausal and 21/42 (50%) postmenopausal subjects. NAF leptin was significantly lower (p = 0.042) in premenopausal than postmenopausal women with a BMI < 25, but not in those with a higher BMI. NAF leptin was significantly associated with BMI in premenopausal (p = 0.011) but not in postmenopausal women. Serum leptin was associated with BMI in both premenopausal and postmenopausal women (p = 0.0001 for both). NAF and serum leptin were associated in premenopausal (p = 0.02) but not postmenopausal women. Neither NAF nor serum leptin was associated with premenopausal or postmenopausal breast cancer. Our findings include that I) leptin is present in the breast and detectable in a subset of NAF samples, 2) NAF leptin in premenopausal but not postmenopausal women parallels serum leptin levels, and 3) neither NAF nor serum levels of leptin were associated with premenopausal or postmenopausal breast cancer.
引用
收藏
页码:336 / 340
页数:5
相关论文
共 23 条
[1]   Regulation of circulating soluble leptin receptor levels by gender, adiposity, sex steroids, and leptin -: Observational and interventional studies in humans [J].
Chan, JL ;
Blüher, S ;
Yiannakouris, N ;
Suchard, MA ;
Kratzsch, J ;
Mantzoros, CS .
DIABETES, 2002, 51 (07) :2105-2112
[2]   Serum immunoreactive leptin concentrations in normal-weight and obese humans [J].
Considine, RV ;
Sinha, MK ;
Heiman, ML ;
Kriauciunas, A ;
Stephens, TW ;
Nyce, MR ;
Ohannesian, JP ;
Marco, CC ;
McKee, LJ ;
Bauer, TL ;
Caro, JF .
NEW ENGLAND JOURNAL OF MEDICINE, 1996, 334 (05) :292-295
[3]   Effects of sex steroid hormones and menopause on serum leptin concentrations [J].
Di Carlo, C ;
Tommaselli, GA ;
Nappi, C .
GYNECOLOGICAL ENDOCRINOLOGY, 2002, 16 (06) :479-491
[4]   Leptin mediates a proliferative response in human MCF7 breast cancer cells [J].
Dieudonne, MN ;
Machinal-Quelin, F ;
Serazin-Leroy, V ;
Leneveu, MC ;
Pecquery, R ;
Giudicelli, Y .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2002, 293 (01) :622-628
[5]   Expression of the long (OB-RB) and short (OB-RA) forms of the leptin receptor throughout the oestrous cycle in the mature rat ovary [J].
Duggal, PS ;
Weitsman, SR ;
Magoffin, DA ;
Norman, RJ .
REPRODUCTION, 2002, 123 (06) :899-905
[6]   Review of anthropometric factors and breast cancer risk [J].
Friedenreich, CM .
EUROPEAN JOURNAL OF CANCER PREVENTION, 2001, 10 (01) :15-32
[7]   Leptin and the regulation of body weight in mammals [J].
Friedman, JM ;
Halaas, JL .
NATURE, 1998, 395 (6704) :763-770
[8]   Transcellular transport of leptin by the short leptin receptor isoform ObRa in Madin-Darby Canine Kidney cells [J].
Hileman, SM ;
Tornoe, J ;
Flier, JS ;
Bjorbæk, C .
ENDOCRINOLOGY, 2000, 141 (06) :1955-1961
[9]   Characterizaton of short isoforms of the leptin receptor in rat cerebral microvessels and of brain uptake of leptin in mouse models of obesity [J].
Hileman, SM ;
Pierroz, DD ;
Masuzaki, H ;
Bjorbæk, C ;
El-Haschimi, K ;
Banks, WA ;
Flier, JS .
ENDOCRINOLOGY, 2002, 143 (03) :775-783
[10]   Difference in leptin mRNA levels between omental and subcutaneous abdominal adipose tissue from obese humans [J].
Hube, F ;
Lietz, U ;
Igel, M ;
Jensen, PB ;
Tornqvist, H ;
Joost, HG ;
Hauner, H .
HORMONE AND METABOLIC RESEARCH, 1996, 28 (12) :690-693