Innovative Electrochemical Approach for an Early Detection of microRNAs

被引:114
作者
Lusi, E. A. [2 ]
Passamano, M. [1 ]
Guarascio, P. [2 ]
Scarpa, A. [1 ]
Schiavo, L. [1 ]
机构
[1] Technobiochip Biol Div, I-80078 Pozzuoli, NA, Italy
[2] St Camillo Hosp Rome, Dept Internal Med, Liver Unit, I-00152 Rome, Italy
关键词
MIR-122; BIOSENSORS; CANCER; VIRUS;
D O I
10.1021/ac8026788
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The recent findings of circulating cell-free tissue specific microRNAs in the systemic circulation and the potential of their use as specific markers of disease highlight the need to make microRNAs testing a routine part of medical care. At the present time, microRNAs are detected by long and laborious techniques such as Northen blot, RT-PCR, and microarrays. The originality of our work consists in performing microRNA detection through an electrochemical genosensor using a label-free method. We were able to directly detect microRNAs without the need of PCR and a labeling reaction. The test is simple, very fast and ultrasensitive, with a detection limit of 0.1 pmol. Particularly feasible for a routine microRNAs detection in serum and other biological samples, our technical approach would be of great scientific value and become a common method for simple miRNAs routine detection in both clinical and research settings.
引用
收藏
页码:2819 / 2822
页数:4
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