Molecular inversion probe-based SPR biosensing for specific, label-free and real-time detection of regional DNA methylation

被引：88

作者：

Carrascosa, Laura G. ^{[1
]}

Ibn Sina, Abu Ali ^{[1
]}

Palanisamy, Ramkumar ^{[1
]}

Sepulveda, Borja ^{[2
,3
]}

Otte, Marinus A. ^{[2
,3
]}

Rauf, Sakandar ^{[1
]}

Shiddiky, Muhamad J. A. ^{[1
]}

Trau, Matt ^{[1
,4
]}

机构：

[1] Univ Queensland, AIBN, Brisbane, Qld 4072, Australia

[2] CSIC, Catalan Inst Nanosci & Nanotechnol ICN2, Bellaterra 08193, Spain

[3] CIBER BBN, Bellaterra 08193, Spain

[4] Univ Queensland, Sch Chem & Mol Biosci, Brisbane, Qld 4072, Australia

来源：

CHEMICAL COMMUNICATIONS | 2014年 / 50卷 / 27期

关键词：

CANCER; PROTEIN; MEDIP; GOLD; GENE;

D O I：

10.1039/c3cc49607d

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

DNA methylation has the potential to be a clinically important biomarker in cancer. This communication reports a real-time and label-free biosensing strategy for DNA methylation detection in the cancer cell line. This has been achieved by using surface plasmon resonance biosensing combined with the highly specific molecular inversion probe based amplification method, which requires only 50 ng of bisulfite treated genomic DNA.

引用

页码：3585 / 3588

页数：4

共 23 条

[1]

Label-free detection of DNA mutations by SPR: application to the early detection of inherited breast cancer [J].

Carrascosa, L. G. ;

Calle, A. ;

Lechuga, L. M. .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2009, 393 (04) :1173-1182

[2]

Targeted bisulfite sequencing reveals changes in DNA methylation associated with nuclear reprogramming [J].

Deng, Jie ;

Shoemaker, Robert ;

Xie, Bin ;

Gore, Athurva ;

LeProust, Emily M. ;

Antosiewicz-Bourget, Jessica ;

Egli, Dieter ;

Maherali, Nimet ;

Park, In-Hyun ;

Yu, Junying ;

Daley, George Q. ;

Eggan, Kevin ;

Hochedlinger, Konrad ;

Thomson, James ;

Wang, Wei ;

Gao, Yuan ;

Zhang, Kun .

NATURE BIOTECHNOLOGY, 2009, 27 (04) :353-360

[3]

Fouse SD, 2010, EPIGENOMICS-UK, V2, P105, DOI [10.2217/epi.09.35, 10.2217/EPI.09.35]

[4]

Characterization of DNA probes immobilized on gold surfaces [J].

Herne, TM ;

Tarlov, MJ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1997, 119 (38) :8916-8920

[5]

DNA methylation profiling in the clinic: applications and challenges [J].

Heyn, Holger ;

Esteller, Manel .

NATURE REVIEWS GENETICS, 2012, 13 (10) :679-692

[6]

Surface plasmon resonance sensors for detection of chemical and biological species [J].

Homola, Jiri .

CHEMICAL REVIEWS, 2008, 108 (02) :462-493

[7]

The use of multiple displacement amplified DNA as a control for methylation specific PCR, pyrosequencing, bisulfite sequencing and methylation-sensitive restriction enzyme PCR [J].

Hughes, Simon ;

Jones, J. Louise .

BMC MOLECULAR BIOLOGY, 2007, 8

[8]

Base-dependent competitive adsorption of single-stranded DNA on gold [J].

Kimura-Suda, H ;

Petrovykh, DY ;

Tarlov, MJ ;

Whitman, LJ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (30) :9014-9015

[9]

Chaperone Probes and Bead-Based Enhancement To Improve the Direct Detection of mRNA Using Silicon Photonic Sensor Arrays [J].

Kindt, Jared T. ;

Bailey, Ryan C. .

ANALYTICAL CHEMISTRY, 2012, 84 (18) :8067-8074

[10]

Microdevices for detecting locus-specific DNA methylation at CpG resolution [J].

Koo, Kevin M. ;

Wee, Eugene J. H. ;

Rauf, Sakandar ;

Shiddiky, Muhammad J. A. ;

Trau, Matt .

BIOSENSORS & BIOELECTRONICS, 2014, 56 :278-285

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