MicroRNA-344 inhibits 3T3-L1 cell differentiation via targeting GSK3β of Wnt/β-catenin signaling pathway

被引：71

作者：

Chen, Hu ^{[1
]}

Wang, Siqi ^{[1
]}

Chen, Luxi ^{[1
]}

Chen, Yaosheng ^{[1
]}

Wu, Ming ^{[1
]}

Zhang, Yun ^{[1
]}

Yu, Kaifan ^{[1
]}

Huang, Zheng ^{[1
]}

Qin, Lijun ^{[1
]}

Mo, Delin ^{[1
]}

机构：

[1] Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510006, Guangdong, Peoples R China

来源：

FEBS LETTERS | 2014年 / 588卷 / 03期

基金：

中国国家自然科学基金;

关键词：

miR-344; GSK3; beta; Adipogenesis; Wnt/beta-catenin signaling; ACTIVATED RECEPTOR-GAMMA; BETA-CATENIN; ANIMAL DEVELOPMENT; KEY REGULATOR; IN-VITRO; ADIPOGENESIS; EXPRESSION; CYCLIN-D1; MECHANISM; GSK3;

D O I：

10.1016/j.febslet.2013.12.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Differentiation of 3T3-L1 cells into adipocytes involves a highly orchestrated series of complex events in which microRNAs might play an essential role. In this study, we found that the overexpression of microRNA-344 (miR-344) inhibits 3T3-L1 cell differentiation and decreases triglyceride accumulation after MDI stimulation. We demonstrated that miR-344 directly targets the 3' UTR of GSK3 beta (Glycogen synthase kinase 3 beta). Knockdown of GSK3 beta with siRNA results in inhibiting 3T3-L1 differentiation, while its overexpression restores the effect of miR-344. In addition, miR-344 elevates the level of active beta-catenin, which is the downstream effector of GSK3 beta in the Wnt/beta-catenin signaling pathway. These data indicate that miR-344 inhibits adipocyte differentiation via targeting GSK3 beta and subsequently activating the Wnt/beta-catenin signaling pathway. Crown Copyright (C) 2013 Published by Elsevier B.V. on behalf of Federation of European Biochemical Society. All rights reserved.

引用

页码：429 / 435

页数：7

共 28 条

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