Detection of the Virulent Form of AVR3a from Phytophthora infestans following Artificial Evolution of Potato Resistance Gene R3a

被引：31

作者：

Chapman, Sean ^{[1
]}

Stevens, Laura J. ^{[1
,2
,3
]}

Boevink, Petra C. ^{[1
,3
]}

Engelhardt, Stefan ^{[2
,3
]}

Alexander, Colin J. ^{[4
]}

Harrower, Brian ^{[1
]}

Champouret, Nicolas ^{[5
]}

McGeachy, Kara ^{[1
]}

Van Weymers, Pauline S. M. ^{[1
,2
,3
]}

Chen, Xinwei ^{[1
,3
]}

Birch, Paul R. J. ^{[1
,2
,3
]}

Hein, Ingo ^{[1
,3
]}

机构：

[1] James Hutton Inst, Dundee, Scotland

[2] Univ Dundee, James Hutton Inst, Div Plant Sci, Invergowrie Dundee, Scotland

[3] Dundee Effector Consortium, Invergowrie Dundee, Scotland

[4] Biomath & Stat Scotland, Invergowrie Dundee, Scotland

[5] JR Simplot Co, Simplot Plant Sci, Boise, ID USA

来源：

PLOS ONE | 2014年 / 9卷 / 10期

基金：

英国生物技术与生命科学研究理事会; 美国食品与农业研究所;

关键词：

LATE BLIGHT RESISTANCE; HYPERSENSITIVE RESPONSE; CELL-DEATH; NUCLEOTIDE-BINDING; EFFECTOR AVR3A; PROTEIN; HOST; TRANSFORMATION; SUPERFAMILY; PERCEPTION;

D O I：

10.1371/journal.pone.0110158

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Engineering resistance genes to gain effector recognition is emerging as an important step in attaining broad, durable resistance. We engineered potato resistance gene R3a to gain recognition of the virulent AVR3a(EM) effector form of Phytophthora infestans. Random mutagenesis, gene shuffling and site-directed mutagenesis of R3a were conducted to produce R3a* variants with gain of recognition towards AVR3a(EM). Programmed cell death following gain of recognition was enhanced in iterative rounds of artificial evolution and neared levels observed for recognition of AVR3a(KI) by R3a. We demonstrated that R3a*-mediated recognition responses, like for R3a, are dependent on SGT1 and HSP90. In addition, this gain of response is associated with re-localisation of R3a* variants from the cytoplasm to late endosomes when co-expressed with either AVR3a(KI) or AVR3a(EM) a mechanism that was previously only seen for R3a upon co-infiltration with AVR3a(KI). Similarly, AVR3a(EM) specifically re-localised to the same vesicles upon recognition by R3a* variants, but not with R3a. R3a and R3a* provide resistance to P. infestans isolates expressing AVR3a(KI) but not those homozygous for AVR3a(EM).

引用

页数：11

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