Construction of amperometric immunosensors based on the electrogeneration of a permeable biotinylated polypyrrole film

被引：65

作者：

Ionescu, RE

Gondran, C

Gheber, LA

Cosnier, S

Marks, RS

机构：

[1] Ben Gurion Univ Negev, Inst Appl Biosci, IL-84105 Beer Sheva, Israel

[2] Ben Gurion Univ Negev, Dept Biotechnol Engn, IL-84105 Beer Sheva, Israel

[3] Univ Grenoble 1, FR CNRS 2607, Inst Chim Mol Grenoble, UMR CNRS 5630,Lab Electrochim Organ & Photochim R, Grenoble, France

来源：

ANALYTICAL CHEMISTRY | 2004年 / 76卷 / 22期

关键词：

D O I：

10.1021/ac049413z

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The construction of amperometric immunosensors to cholera antitoxin immunoglobulins were shown to have improved sensitivity when the cholera toxin B subunit biorecognition entity was linked to an electrogenerated biotinylated polypyrrole film copolymerized with pyrrolelactobionamide monomer. The copolymer exhibits greater film permeability than biotinylated polypyrrolic or polyphenolic films for the permeation of electroactive species. Hence, when the presence of the HRP marker of the immunoassay was determined using hydroquinone, the production of electroactive quinone was shown to permeate faster to the electrode, thus providing a faster response time.

引用

页码：6808 / 6813

页数：6

共 47 条

[1] Validation of antibody-based recognition by piezoelectric transducers through electroacoustic admittance analysis [J].