Development of EIS cell chips and their application for cell analysis

被引：17

作者：

Primiceri, Elisabetta ^{[1
]}

Chiriaco, Maria Serena ^{[1
]}

Ionescu, Rodica Elena ^{[1
]}

D'Amone, Eliana ^{[1
]}

Cingolani, Roberto ^{[1
]}

Rinaldi, Ross ^{[1
]}

Maruccio, Giuseppe ^{[1
]}

机构：

[1] CNR INFM, Natl Nanotechnol Lab, I-73100 Lecce, Italy

来源：

MICROELECTRONIC ENGINEERING | 2009年 / 86卷 / 4-6期

关键词：

Cell Chip; Impedance Spectroscopy; Cell counting; Lab on chip; Nanotechnology; IMPEDANCE SPECTROSCOPY; CYTOTOXICITY; ELECTRODES; ADHESION; SURFACES; MONITOR;

D O I：

10.1016/j.mee.2008.12.026

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

We report the development of EIS cell chips able to monitor cell growth and adhesion. They are made of transparent or semitransparent materials to allow complementary analysis of cell behaviour during the measurements through optical microscopy. Our approach is cheap both in fabrication and usage, it is not invasive for cells and it does not require any additional reagent. Our devices are particular suitable to count cells or to evaluate cell morphology and changes as a consequence of different treatments. (C) 2009 Published by Elsevier B.V.

引用

页码：1477 / 1480

页数：4

共 15 条

[1] Bioelectrical impedance assay to monitor changes in cell shape during apoptosis [J].

Arndt, S ;

Seebach, J ;

Psathaki, K ;

Galla, HJ ;

Wegener, J .

BIOSENSORS & BIOELECTRONICS, 2004, 19 (06) :583-594

[2] Impedance analysis of renal vascular smooth muscle cells [J].

Balasubramanian, Lavanya ;

Yip, Kay-Pong ;

Hsu, Tai-Hsin ;

Lo, Chun-Min .

AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2008, 295 (04) :C954-C965

[3] Evaluation of endothelial cell adhesion onto different protein/gold electrodes by EIS [J].

Bouafsoun, Amira ;

Helali, Saloua ;

Othmane, Ali ;

Kerkeni, Abdelhamid ;

Prigent, Anne-France ;

Jaffrezic-Renault, Nicole ;

Bessueille, Francois ;

Leonard, Didier ;

Ponsonnet, Laurence .

MACROMOLECULAR BIOSCIENCE, 2007, 7 (05) :599-610

[4] Real-time monitoring approach: Assessment of effects of antibodies on the adhesion of NCI-H460 cancer cells to the extracellular matrix [J].

Chen, Yu ;

Zhang, Juntao ;

Wang, Yanping ;

Zhang, Li ;

Julien, Reboud ;

Tang, Kumcheong ;

Balasubramanian, Narayanan .

BIOSENSORS & BIOELECTRONICS, 2008, 23 (09) :1390-1396

[5] Cell detection and counting through cell lysate impedance spectroscopy in microfluidic devices [J].

Cheng, Xuanhong ;

Liu, Yi-shao ;

Irimia, Daniel ;

Demirci, Utkan ;

Yang, Liju ;

Zamir, Lee ;

Rodriguez, William R. ;

Toner, Mehmet ;

Bashir, Rashid .

LAB ON A CHIP, 2007, 7 (06) :746-755

[6] Monitoring of cellular behaviour by impedance measurements on interdigitated electrode structures [J].

Ehret, R ;

Baumann, W ;

Brischwein, M ;

Schwinde, A ;

Stegbauer, K ;

Wolf, B .

BIOSENSORS & BIOELECTRONICS, 1997, 12 (01) :29-41

[7] MICROMOTION OF MAMMALIAN-CELLS MEASURED ELECTRICALLY [J].

GIAEVER, I ;

KEESE, CR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (17) :7896-7900

[8] Probing biomolecular interactions at conductive and semiconductive surfaces by impedance spectroscopy: Routes to impedimetric immunosensors, DNA-Sensors, and enzyme biosensors [J].

Katz, E ;

Willner, I .

ELECTROANALYSIS, 2003, 15 (11) :913-947

[9] A BIOSENSOR THAT MONITORS CELL MORPHOLOGY WITH ELECTRICAL FIELDS [J].

KEESE, CR ;

GIAEVER, I .

IEEE ENGINEERING IN MEDICINE AND BIOLOGY MAGAZINE, 1994, 13 (03) :402-408

[10] Assessment of cytotoxicity of quantum dots and gold nanoparticles using cell-based. impedance spectroscopy [J].

Male, Keith B. ;

Lachance, Bernard ;

Hrapovic, Sabahudin ;

Sunahara, Geoff ;

Luong, John H. T. .

ANALYTICAL CHEMISTRY, 2008, 80 (14) :5487-5493

← 1 2 →