A high-throughput screening assay for hydroxynitrile lyase activity

被引:18
作者
Andexer, Jennifer [1 ]
Guterl, Jan-Karl [1 ]
Pohl, Martina [1 ]
Eggert, Thorsten [1 ]
机构
[1] Univ Dusseldorf, KFA Julich, Forschungszentrum, Inst Mol Enzyme Technol, D-52426 Julich, Germany
关键词
D O I
10.1039/b607863j
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A high-throughput screening assay for hydroxynitrile lyase activity accepting a wide range of HNL-substrates is presented, which is useful either for enzyme fingerprinting or screening of huge variant libraries generated in metagenome or directed evolution approaches.
引用
收藏
页码:4201 / 4203
页数:3
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