Toward Multiprotein Nanoarrays Using Nanografting and DNA Directed Immobilization of Proteins

被引：58

作者：

Bano, Fouzia ^{[2
,3
]}

Fruk, Ljiljana ^{[1
]}

Sanavio, Barbara ^{[2
,3
]}

Glettenberg, Maximilian ^{[1
]}

Casalls, Loredana ^{[3
,4
]}

Niemeyer, Christof M. ^{[1
]}

Scoles, Giacinto ^{[2
,3
,4
]}

机构：

[1] Tech Univ Dortmund, Fac Chem, D-44221 Dortmund, Germany

[2] Scuola Int Super Studi Avanzati, I-34014 Trieste, Italy

[3] Sincrotrone Trieste, ELETTRA, I-34012 Trieste, Italy

[4] IIT, SISSA Unit, I-34014 Trieste, Italy

来源：

NANO LETTERS | 2009年 / 9卷 / 07期

关键词：

SELF-ASSEMBLED MONOLAYERS; HORSERADISH-PEROXIDASE; NANOSTRUCTURES; MICROARRAYS; GENERATION; ARRAYS;

D O I：

10.1021/nl9008869

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Atomic force microscopy nanografting was utilized to prepare DNA nanopatches of different sizes (200 x 200 to 1000 x 1000 nm(2)) onto which DNA-protein conjugates can be anchored through DNA-directed immobilization. Height measurements were used to assess the binding of the proteins as well as their subsequent interaction with other components, such as antibodies. The results indicate that nanografted patch arrays are well suited for application in blosensing and could enable the fabrication of multifeature protein nanoarrays.

引用

页码：2614 / 2618

页数：5

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