Strontium (Sr) and silver (Ag) loaded nanotubular structures with combined osteoinductive and antimicrobial activities

被引：145

作者：

Cheng, Hao ^{[1
,2
,3
]}

Xiong, Wei ^{[1
]}

Fang, Zhong ^{[1
]}

Guan, Hanfeng ^{[1
]}

Wu, Wei ^{[1
]}

Li, Yong ^{[1
]}

Zhang, Yong ^{[1
]}

Alvarez, Mario Moises ^{[2
,3
,4
,5
]}

Gao, Biao ^{[6
]}

Huo, Kaifu ^{[6
]}

Xu, Jiangwen ^{[6
]}

Xu, Na ^{[6
]}

Zhang, Chengcheng ^{[6
]}

Fu, Jijiang ^{[6
]}

Khademhosseini, Ali ^{[2
,3
,5
,7
,8
,9
]}

Li, Feng ^{[1
]}

机构：

[1] Huazhong Univ Sci & Technol, Tongji Med Coll, Tongji Hosp, Othopead Dept, 1095 Jiefang Ave, Wuhan 430030, Peoples R China

[2] Harvard Univ, Brigham & Womens Hosp, Biomat Innovat Res Ctr, Div Biomed Engn,Dept Med,Med Sch, Cambridge, MA 02139 USA

[3] MIT, Harvard Mit Div Hlth Sci & Technol, Cambridge, MA 02139 USA

[4] Ctr Biotecnol FEMSA, Tecnol Monterrey, Ave Eugenio Garza Sada 2501 Sur Col Tecnol, Monterrey 64849, Nuevo Leon, Mexico

[5] MIT, Microsyst Technol Labs, Cambridge, MA 02139 USA

[6] Wuhan Univ Sci & Technol, Sch Met & Mat, State Key Lab Refractories & Met, Wuhan 430081, Peoples R China

[7] Harvard Univ, Wyss Inst Biol Inspired Engn, Boston, MA 02115 USA

[8] Konkuk Univ, Coll Anim Biosci & Technol, Dept Bioind Technol, Seoul 143701, South Korea

[9] King Abdulaziz Univ, Dept Phys, Jeddah 21569, Saudi Arabia

来源：

ACTA BIOMATERIALIA | 2016年 / 31卷

基金：

中国国家自然科学基金;

关键词：

Strontium; Silver; Titanium; Nanotubular structures; Antibacterial; Osteogenic; IN-VITRO; BONE STRENGTH; OSTEOBLAST FUNCTIONS; COMPUTED-TOMOGRAPHY; OSTEOGENIC ACTIVITY; BACTERIAL ADHESION; TITANIUM SURFACES; OSTEOPOROTIC RATS; TIO2; NANOTUBES; ANTIBACTERIAL;

D O I：

10.1016/j.actbio.2015.11.046

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

Two frequent problems are associated with the titanium surfaces of bone/dental implants: lack of native tissue integration and associated infection. These problems have prompted a significant body of research regarding the modification of these surfaces. The present study describes a hydrothermal treatment for the fabrication of strontium (Sr) and silver (Ag) loaded nanotubular structures with different tube diameters on titanium surfaces. The Sr loading from a Sr(OH)(2) solution was regulated by the size of the inner diameter of the titanium nanotubes (NT) (30 nm or 80 nm, formed at 10 V or 40 V, respectively). The quantity of Ag was adjusted by immersing the samples in 1.5 or 2.0 M AgNO3 solutions. Sr and Ag were released in a controllable and prolonged matter from the NT-Ag.Sr samples, with negligible cytotoxicity. Prominent antibacterial activity was observed due to the release of Ag. Sr incorporation enhanced the initial cell adhesion, migration, and proliferation of preosteoblast MC3T3-E1 cells. Sr release also up-regulated the expression of osteogenic genes and induced mineralization, as suggested by the presence of more mineralized calcium nodules in cells cultured on NT-Ag.Sr surfaces. In vivo experiments showed that the Sr-loaded samples accelerated the formation of new bone in both osteoporosis and bone defect models, as confirmed by X-ray, Micro-CT evaluation, and histomorphometric analysis of rats implanted with NT-Ag.Sr samples. The antibacterial activity and outstanding osteogenic properties of NT-Ag.Sr samples highlight their excellent potential for use in clinical applications. Statement of Significance Two frequent problems associated with Ti surfaces, widely used in orthopedic and dental arenas, are their lack of native tissue integration and risk of infection. We describe a novel approach for the fabrication of strontium (Sr) and silver (Ag) loaded nanotubular structures on titanium surfaces. A relevant aspect of this work is the demonstration of long-lasting and controllable Ag release, leading to excellent antibacterial and anti-adherent properties against methicillin-resistant Staphylococcus aureus (MRSA), and Gram-negative bacteria such as Escherichia coli. The extended release of Sr accelerates the filling of bone defects by improving the repair of damaged cortical bone and increasing trabecular bone microarchitecture. Our results highlight the potential of Sr and Ag loaded nanotubular structures for use in clinical applications. (C) 2015 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

引用

页码：388 / 400

页数：13

共 60 条

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