Expression of junction-associated proteins differentiates mouse intestinal M cells from enterocytes

被引：29

作者：

Clark, AM ^{[1
]}

Hirst, BH ^{[1
]}

机构：

[1] Univ Newcastle, Dept Physiol Sci, Sch Med, Newcastle Upon Tyne NE2 4HH, Tyne & Wear, England

来源：

HISTOCHEMISTRY AND CELL BIOLOGY | 2002年 / 118卷 / 02期

基金：

英国惠康基金;

关键词：

follicle-associated epithelium; M cell; junctional complex; adherens junction; beta-catenin;

D O I：

10.1007/s00418-002-0433-z

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The intestinal lymphoid follicles and associated structures are specialised antigen sampling and inductive sites of the mucosal immune system. The overlying follicle-associated epithelium (FAE) includes the specialised antigen sampling M cells that are also exploited as a route of pathogen invasion. In this immunohistochemical study we analysed the junctional complexes of the mouse intestinal FAE. Protein expression at this site resembled that of other simple epithelia. Specifically, claudin-1/3 and ZO-1 were detected in the tight junctions, E-cadherin, alpha-, beta- and gamma-catenin, vinculin, alpha-actinin and polymerised actin were associated with the adherens junctions and the desmosomes were labelled with a desmosomal protein probe. These markers failed to reveal cell type-associated variations in the tight junctions and desmosomes. In contrast, M cell adherens junctions were distinguished by enhanced expression of beta-catenin, alpha-actinin, polymerised actin and, in some areas, E-cadherin. In addition, M cell junctions exhibited increased expression of intercellular adhesion molecule-1 and phosphotyrosine, and the M cell apical surfaces displayed characteristic patterns of beta-catenin, alpha-actinin and actin expression. We have thus partially defined the junctional complexes of mouse intestinal FAE and identified M cell-specific characteristics that may further explain the biology and function of this unique cell type.

引用

页码：137 / 147

页数：11

共 38 条

[21]

Hatzfeld M, 1999, INT REV CYTOL, V186, P179

[22] The roles of claudin superfamily proteins in paracellular transport [J].

Heiskala, M ;

Peterson, PA ;

Yang, Y .

TRAFFIC, 2001, 2 (02) :92-98

[23] Infection of human intestinal epithelial cells with invasive bacteria upregulates apical intercellular adhesion molecule-1 (ICAM-1) expression and neutrophil adhesion [J].

Huang, GTJ ;

Eckmann, L ;

Savidge, TC ;

Kagnoff, MF .

JOURNAL OF CLINICAL INVESTIGATION, 1996, 98 (02) :572-583

[24] Studying M cells and their role in infection [J].

Jepson, MA ;

Clark, MA .

TRENDS IN MICROBIOLOGY, 1998, 6 (09) :359-365

[25] The role of M cells in Salmonella infection [J].

Jepson, MA ;

Clark, MA .

MICROBES AND INFECTION, 2001, 3 (14-15) :1183-1190

[26]

JEPSON MA, 2001, M CELLS THEIR ROLE I

[27] Cytosolic distribution of villin in M cells from mouse Peyer's patches correlates with the absence of a brush border [J].

Kerneis, S ;

Bogdanova, A ;

ColucciGuyon, E ;

Kraehenbuhl, JP ;

Pringault, E .

GASTROENTEROLOGY, 1996, 110 (02) :515-521

[28] Epithelial M cells: Differentiation and function [J].

Kraehenbuhl, JP ;

Neutra, MR .

ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 2000, 16 :301-332

[29]

MADARA JL, 1984, GASTROENTEROLOGY, V87, P1091

[30] Unmasking of intestinal epithelial lateral membrane beta 1 integrin consequent to transepithelial neutrophil migration in vitro facilitates inv-mediated invasion by Yersinia pseudotuberculosis [J].

McCormick, BA ;

Nusrat, A ;

Parkos, CA ;

DAndrea, L ;

Hofman, PM ;

Carnes, D ;

Liang, TW ;

Madara, JL .

INFECTION AND IMMUNITY, 1997, 65 (04) :1414-1421

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