DNA sequencing by capillary electrophoresis (review)

被引：55

作者：

Dolník, V ^{[1
]}

机构：

[1] Mol Dynam, Sunnyvale, CA 94086 USA

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1999年 / 41卷 / 2-3期

关键词：

DNA sequencing; capillary electrophoresis; sieving matrix; entangled polymer; wall coating;

D O I：

10.1016/S0165-022X(99)00041-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA sequencing by capillary electrophoresis has been reviewed with an emphasis on progress during the last four years. The effects of sample purification, composition of sieving matrices, electric field strength, temperature, wall coating and DNA labeling on the DNA sequencing performance are discussed. Multicapillary array instrumentation is compared with one-capillary systems. Integrated systems that perform the whole DNA sequencing operation online starting from the DNA amplification through base calling and data processing are discussed. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：103 / 119

页数：17

共 112 条

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