Radial capillary array electrophoresis microplate and scanner for high-performance nucleic acid analysis

被引:245
作者
Shi, YN
Simpson, PC
Scherer, JR
Wexler, D
Skibola, C
Smith, MT
Mathies, RA [1 ]
机构
[1] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA
[2] Univ Calif Berkeley, Sch Publ Hlth, Berkeley, CA 94720 USA
关键词
D O I
10.1021/ac990518p
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The design, fabrication, and operation of a radial capillary array electrophoresis microplate and scanner for high-throughput DNA analysis is presented. The microplate consists of a central common anode reservoir coupled to 96 separate microfabricated separation channels connected to sample injectors on the perimeter of the 10cm-diameter wafer. Detection is accomplished by a laser-excited rotary confocal scanner with four color detection channels. Loading of 96 samples in parallel is achieved using a pressurized capillary array system. High-quality separations of 96 pBR322 restriction digest samples are achieved in <120 s with the microplate system. The practical utility and multicolor detection capability is demonstrated by analyzing 96 methylenetetrahydrofolate reductase (MTHFR) alleles in parallel using a noncovalent 2-color staining method. This work establishes the feasibility of performing high-throughput genotyping separations with capillary array electrophoresis microplates.
引用
收藏
页码:5354 / 5361
页数:8
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