Role of microRNA-143 in Fas-mediated apoptosis in human T-cell leukemia Jurkat cells

被引:86
作者
Akao, Yukihiro [1 ,3 ]
Nakagawa, Yoshihito [1 ]
Iio, Akio [1 ]
Naoe, Tomoki [2 ]
机构
[1] Gifu Int Inst Biotechnol, Dept Med Oncol, Gifu 5040838, Japan
[2] Nagoya Univ, Grad Sch Med, Dept Hematol & Oncol, Showa Ku, Nagoya, Aichi 4668550, Japan
[3] Gifu Univ, United Grad Sch Drug Discovery & Med Informat Sci, Gifu 5011193, Japan
关键词
MiRNA-143; Apoptosis; Fas; Jurkat T-cell; ERK5; CYTOCHROME-C; CANCER; FAMILY; DEATH; ACTIVATION; EXPRESSION; MECHANISM; CASPASE-8; PROTEINS; AND-145;
D O I
10.1016/j.leukres.2009.04.019
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Treatment of Jurkat T cells with Fas-activating antibody (CH-11) facilitated rapid cell death that was shown to be caspase-dependent apoptosis. The expression of miR-143 was up-regulated during the apoptosis with time. The increased expression of miR-143 emerged from 1 to 2 h after the treatment, at which time the caspases-8 and -3 were also activated; and this increase was almost canceled by the pretreatment with an inhibitor of caspase-3 or -8. Furthermore, the transfection of Jurkat cells with mature miR-143 induced a significant growth suppression and enhancement of CH-11-induced apoptosis. On the contrary, an extracellular signal-regulated protein kinase 5 (ERK5), which was determined to be a target of miR-143 in colon cancer DLD-1 cells, was time-dependently down-regulated at the translational level after the treatment. During the apoptosis, the expression level of FasL was maintained and the level of nuclear-Foxo3a was increased in the early phase. These data suggest that the up-regulation of miR-143 could be related to the apoptosis in part by targeting ERK5, which leads to promotion of Foxo3a/FasL positive feedback loop. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1530 / 1538
页数:9
相关论文
共 27 条
  • [1] AKAO Y, 1990, CANCER RES, V50, P4856
  • [2] Downregulation of microRNAs-143 and-145 in B-cell malignancies
    Akao, Yukihiro
    Nakagawa, Yoshihito
    Kitade, Yukio
    Kinoshita, Tomohiro
    Naoe, Tomoki
    [J]. CANCER SCIENCE, 2007, 98 (12) : 1914 - 1920
  • [3] MicroRNA-143 and-145 in colon cancer
    Akao, Yukihiro
    Nakagawa, Yoshihito
    Naoe, Tomoki
    [J]. DNA AND CELL BIOLOGY, 2007, 26 (05) : 311 - 320
  • [4] Akao Y, 2006, ONCOL REP, V16, P845
  • [5] Protein phosphatase 2A regulates apoptosis in neutrophils by dephosphorylating both p38 MAPK and its substrate caspase 3
    Alvarado-Kristensson, M
    Andersson, T
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (07) : 6238 - 6244
  • [6] MicroRNA functions in animal development and human disease
    Alvarez-Garcia, I
    Miska, EA
    [J]. DEVELOPMENT, 2005, 132 (21): : 4653 - 4662
  • [7] MicroRNA pathways in flies and worms: Growth, death, fat, stress, and timing
    Ambros, V
    [J]. CELL, 2003, 113 (06) : 673 - 676
  • [8] The functions of animal microRNAs
    Ambros, V
    [J]. NATURE, 2004, 431 (7006) : 350 - 355
  • [9] MicroRNAs: Genomics, biogenesis, mechanism, and function (Reprinted from Cell, vol 116, pg 281-297, 2004)
    Bartel, David P.
    [J]. CELL, 2007, 131 (04) : 11 - 29
  • [10] Proteolytic regulation of Forkhead transcription factor FOXO3a by caspase-3-like proteases
    Charvet, C
    Alberti, I
    Luciano, F
    Jacquel, A
    Bernard, A
    Auberger, P
    Deckert, M
    [J]. ONCOGENE, 2003, 22 (29) : 4557 - 4568