Protein detecting arrays based on cationic polythiophene-DNA-aptamer complexes

被引：100

作者：

Aberem, Maite Bera

Najari, Ahmed

Ho, Hoang-Anh

Gravel, Jean-Francois

Nobert, Philippe

Boudreau, Denis

Leclerc, Mario ^{[1
]}

机构：

[1] Univ Laval, Dept Chim, Canada Res Chair Electroact & Photoact Polymers, Quebec City, PQ G1K 7P4, Canada

[2] Univ Laval, Dept Chim, Ctr Opt Photon & Laser, Quebec City, PQ G1K 7P4, Canada

来源：

ADVANCED MATERIALS | 2006年 / 18卷 / 20期

关键词：

D O I：

10.1002/adma.200601651

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

aptamer with a cationic polythiophene optical transducer, human thrombin can be specifically detected on microarrays in the attomole range in less than one hour without any tagging of the target. The system can be modified and utilized as a probe for the detection of various proteins or other biomolecules. This work opens new interesting possibilities for simple and rapid multiparametric analysis in genomics and proteomics.

引用

页码：2703 / +

页数：6

共 19 条

[11] 2-I
[12] Label-free electrochemical detection of protein based on a ferrocene-bearing cationic polythiophene and aptamer
Le Floch, Fabien
Ho, Hoang A.
Leclerc, Mario
[J]. ANALYTICAL CHEMISTRY, 2006, 78 (13) : 4727 - 4731
[13] Self-contained, fully integrated biochip for sample preparation, polymerase chain reaction amplification, and DNA microarray detection
Liu, RH
Yang, JN
Lenigk, R
Bonanno, J
Grodzinski, P
[J]. ANALYTICAL CHEMISTRY, 2004, 76 (07) : 1824 - 1831
[14] Chip and solution detection of DNA hybridization using a luminescent zwitterionic polythiophene derivative
Nilsson, KPR
Inganäs, O
[J]. NATURE MATERIALS, 2003, 2 (06) : 419 - U10
[15] Reagentless, reusable, ultrasensitive electrochemical molecular beacon aptasensor
Radi, AE
Sánchez, JLA
Baldrich, E
O'Sullivan, CK
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (01) : 117 - 124
[16] Long-range resonance energy transfer in molecular systems
Scholes, GD
[J]. ANNUAL REVIEW OF PHYSICAL CHEMISTRY, 2003, 54 : 57 - 87
[17] Scanometric DNA array detection with nanoparticle probes
Taton, TA
Mirkin, CA
Letsinger, RL
[J]. SCIENCE, 2000, 289 (5485) : 1757 - 1760
[18] SYSTEMATIC EVOLUTION OF LIGANDS BY EXPONENTIAL ENRICHMENT - RNA LIGANDS TO BACTERIOPHAGE-T4 DNA-POLYMERASE
TUERK, C
GOLD, L
[J]. SCIENCE, 1990, 249 (4968) : 505 - 510
[19] Label-free electronic detection of thrombin in blood serum by using an aptamer-based sensor
Xiao, Y
Lubin, AA
Heeger, AJ
Plaxco, KW
[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2005, 44 (34) : 5456 - 5459

← 1 2 →